Beclin 1, Autophagy Related (BECN1) (AA 150-300) antibody

Details for Product No. ABIN259945
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Antigen
Synonyms beclin-1, atg6, vps30, beclin1, Beclin-1, becn1, zgc:73097, ATG6, VPS30, 4921513J16Rik, 5430417M23Rik, Atg6
Epitope
AA 150-300
(25), (13), (9), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Chicken, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(213), (87), (60), (32), (30), (21), (18), (15), (12), (6), (2), (1), (1)
Host
Rabbit
(163), (56), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (9), (9), (9), (8), (8), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(187), (85), (69), (54), (39), (31), (16), (16), (16), (10), (5), (1)
Pubmed 5 references available
Quantity 0.05 mL
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Catalog No. ABIN259945
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Immunogen Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300).
Cross-Reactivity (Details) Canine reactivity reported in scientific literature (PMID: 24027311). Possible reactivity to Chicken (87%), Xenopus laevis (79%),Xenopus tropicalis (77%) (not yet validated).
Purification affinity purified
Alternative Name Beclin-1
Background Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumorsuppressor and antiviral function. Autophagy, a process of bulk protein degradationthrough an autophagosomic-lysosomal pathway, is important for differentiation, survivalduring nutrient deprivation, and normal growth control, and is often defective in tumorcells. Beclin 1 was originally isolated in a yeast two hybrid screen to identifyBcl-2-binding partners and maps to a tumor susceptibility locus on human chromosome17q21 that is frequently monoallelically deleted in human breast, ovarian and prostatecancer. Beclin 1 encodes an evolutionarily conserved 60 kDa coiled coil protein that isexpressed in human muscle, epithelial cells and neurons. In gene transfer studies,beclin 1 promotes nutrient deprivation-induced autophagy, inhibits mammarytumorigenesis, and inhibits viral replication. Expression of the Beclin 1 protein isfrequently decreased in malignant breast epithelial cells. Based upon theseobservations, it is speculated that beclin 1 may work through induction of autophagy tonegatively regulate tumorigenesis and to control viral infections. Beclin 1 may also playa role in other biological processes in which autophagy is important such as celldifferentiation and nutritional stress responses. Alternate Names: anti-CTIP-1 COUP-TF interacting protein 1 antibody, anti-Beclin1 autophagy antibody,anti-Coiled-coil myosin-like BCL2-interacting protein antibody, anti-Beclin-1 antibody,anti-Protein GT197 antibody, anti-APG6 antibody, anti-Beclin 1 antibody, anti-Beclin-1antibody.
Gene Symbol: BECN1
Gene ID 8678, 56208, 114558
UniProt Q14457
Application Notes This Beclin 1 antibody is useful for Immunohistochemistry paraffin sections, Immunocytochemistry/Immunofluorescence, and Western blot where a band is seen ~51 kDa. Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry 1:400, Immunohistochemistry-Paraffin 1:400, Immunoprecipitation, Western Blot 1:10000
Protocol Protocol specific for Beclin 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Beclin1 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, 0.1 % BSA, 50 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Beclin 1, Autophagy Related (BECN1) (AA 150-300) antibody Western Blot: Beclin 1 Antibody [ABIN259945] - Detection of Beclin 1 in HeLa whole cell lysate using ABIN259945 at 1:10,000.
anti-Beclin 1, Autophagy Related (BECN1) (AA 150-300) antibody (2) Immunohistochemistry-Paraffin: Beclin 1 Antibody [ABIN259945] - Staining of normal breast tissue using ABIN259945 (20x).
anti-Beclin 1, Autophagy Related (BECN1) (AA 150-300) antibody (3) Immunohistochemistry-Paraffin: Beclin 1 Antibody [ABIN259945] - Staining of breast carcinoma using ABIN259945 (20x).
General Liang, Kleeman, Jiang et al.: "Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein." in: Journal of virology, Vol. 72, Issue 11, pp. 8586-96, 1998 (PubMed).

Aita, Liang, Murty et al.: "Cloning and genomic organization of beclin 1, a candidate tumor suppressor gene on chromosome 17q21." in: Genomics, Vol. 59, Issue 1, pp. 59-65, 1999 (PubMed).

Liang, Jackson, Seaman et al.: "Induction of autophagy and inhibition of tumorigenesis by beclin 1." in: Nature, Vol. 402, Issue 6762, pp. 672-6, 2000 (PubMed).

Massey, Kaushik, Sovak et al.: "Consequences of the selective blockage of chaperone-mediated autophagy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 15, pp. 5805-10, 2006 (PubMed).

Pattingre, Bauvy, Carpentier et al.: "Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2719-28, 2009 (PubMed).

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