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Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300)

Details for Product anti-BECN1 Antibody No. ABIN259945, Supplier: Log in to see
Antigen
  • beclin-1
  • atg6
  • vps30
  • beclin1
  • Beclin-1
  • becn1
  • zgc:73097
  • ATG6
  • VPS30
  • 4921513J16Rik
  • 5430417M23Rik
  • Atg6
  • beclin 1, autophagy related
  • beclin 1
  • beclin 1 protein
  • beclin-1
  • Beclin-1
  • beclin 1 (coiled-coil, myosin-like BCL2 interacting protein)
  • beclin-1-like
  • BECN1
  • becn1
  • atg6
  • CpipJ_CPIJ011087
  • PTRG_07825
  • SJAG_01876
  • Becn1
  • LOC100352353
Epitope
AA 150-300, Internal Region
34
29
20
15
15
15
13
12
12
12
12
11
10
9
8
8
8
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Primate
437
153
143
30
23
15
15
14
12
10
6
6
3
2
2
2
1
1
1
Host
Rabbit
329
115
3
Clonality
Polyclonal
Conjugate
This Beclin 1 antibody is un-conjugated
23
23
23
19
19
19
8
7
7
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
351
177
161
112
101
72
61
27
26
23
13
6
3
1
1
1
Options
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Immunogen Synthetic peptide made to an internal portion of human Beclin 1 (within residues 150-300). [Swiss-Prot# Q14457]
Purification Immunogen affinity purified
Alternative Name Beclin 1 (BECN1 Antibody Abstract)
Background Gene Symbol: BECN1
Molecular Weight Theoretical MW: 51 kDa
Gene ID 8678
UniProt Q14457
Research Area Transcription Factors, Autophagy, Cancer
Application Notes Western Blot 1:10000, Simple Western 1:12.5, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin 1:400This Beclin 1 antibody is useful for Immunohistochemistry paraffin sections, Immunocytochemistry/Immunofluorescence, and Western blot where a band is seen approx. 51 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Beclin 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-Beclin1 primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS, 0.1 % BSA, and 50 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Western Blot: Beclin 1 Antibody [ABIN259945] - Detection of Beclin 1 in HeLa whole ce...
Immunohistochemistry (IHC) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Immunohistochemistry-Paraffin: Beclin 1 Antibody [ABIN259945] - Staining of normal br...
Immunohistochemistry (IHC) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Immunohistochemistry-Paraffin: Beclin 1 Antibody [ABIN259945] - Staining of breast ca...
Simple Western (SimWes) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Simple Western: Beclin 1/ATG6 Antibody [ABIN2599458] - Simple Western lane view shows...
Western Blotting (WB) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Western Blot: Beclin 1/ATG6 Antibody [ABIN2599458] - Beclin 1 antibody image from ver...
Immunofluorescence (IF) image for anti-Beclin 1 antibody (Beclin 1, Autophagy Related) (AA 150-300) (ABIN259945) Immunocytochemistry/Immunofluorescence: Beclin 1/ATG6 Antibody [ABIN2599458] - Beclin...
Product cited in: Choi, Kim, Ham, Choi, Lee, Shin: "Amber Light (590 nm) Induces the Breakdown of Lipid Droplets through Autophagy-Related Lysosomal Degradation in Differentiated Adipocytes." in: Scientific reports, Vol. 6, pp. 28476, 2016 (PubMed).

Pei, Deng, Ye, Wang, Gou, Liu, Zhao, Liao, Yi, Chen: "Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells." in: Autophagy, Vol. 12, Issue 10, pp. 1738-1758, 2016 (PubMed). (Sample species: Pig (Porcine)). Further details: Western Blotting

Lin: "In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy." in: Chemical research in toxicology, Vol. 28, Issue 2, pp. 202-15, 2016 (PubMed).

Song, Sun, Peluso, Zeng, Yu, Lu, Xu, Wang, Liu, Huang, Chen, Durairajan, Zhang, Zhou, Zhang, Lu, Ballabio, Medina, Guo, Li: "A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition." in: Autophagy, Vol. 12, Issue 8, pp. 1372-89, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Barnard, Regan, Hansen, Maycotte, Thorburn, Gustafson: "Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease." in: The Journal of pharmacology and experimental therapeutics, Vol. 358, Issue 2, pp. 282-93, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Tan, Wang, Liang, Chuang: "Human Rad23A plays a regulatory role in autophagy." in: Biochemical and biophysical research communications, Vol. 478, Issue 4, pp. 1772-9, 2016 (PubMed).

Wang, Lin, Chang, Liu, Li, Kuo, Hsieh, Lee: "Hinokitiol induces autophagy in murine breast and colorectal cancer cells." in: Environmental toxicology, Vol. 31, Issue 1, pp. 77-84, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Yao, Zheng, Li, Liu, Shan: "Interference of Notch1 inhibits the growth of glioma cancer cells by inducing cell autophagy and down-regulation of Notch1-Hes-1 signaling pathway." in: Medical oncology (Northwood, London, England), Vol. 32, Issue 6, pp. 610, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Lin, Jan, Kuo: "Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine." in: Molecular pharmaceutics, Vol. 12, Issue 8, pp. 2858-68, 2015 (PubMed).

Lee, Chen, Su, Chueh: "Sirtuin 1 (SIRT1) Deacetylase Activity and NAD⁺/NADH Ratio Are Imperative for Capsaicin-Mediated Programmed Cell Death." in: Journal of agricultural and food chemistry, Vol. 63, Issue 33, pp. 7361-70, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Nah, Pyo, Jung, Yoo, Kam, Chang, Han, Soo A An, Onodera, Jung: "BECN1/Beclin 1 is recruited into lipid rafts by prion to activate autophagy in response to amyloid β 42." in: Autophagy, Vol. 9, Issue 12, pp. 2009-21, 2014 (PubMed).

Lin, Jan, Kuo: "Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine." in: Molecular pharmaceutics, Vol. 11, Issue 9, pp. 3002-8, 2014 (PubMed).

Zhirnov, Klenk: "Influenza A virus proteins NS1 and hemagglutinin along with M2 are involved in stimulation of autophagy in infected cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13107-14, 2013 (PubMed). Further details: Western Blotting

Romao, Gasser, Becker, Guhl, Bajagic, Vanoaica, Ziegler, Roesler, Dengjel, Reichenbach, Münz: "Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing." in: The Journal of cell biology, Vol. 203, Issue 5, pp. 757-66, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Baldo, Soylu, Petersén: "Maintenance of basal levels of autophagy in Huntington's disease mouse models displaying metabolic dysfunction." in: PLoS ONE, Vol. 8, Issue 12, pp. e83050, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Song, Lu, Liu, Chen, Durairajan, Yue, Zhang, Li: "HMGB1 is involved in autophagy inhibition caused by SNCA/α-synuclein overexpression: a process modulated by the natural autophagy inducer corynoxine B." in: Autophagy, Vol. 10, Issue 1, pp. 144-54, 2013 (PubMed). Method employed by authors: Western Blotting (WB)

Pei, Zhao, Ye, Gou, Wang, Yi, Dong, Liu, Luo, Liao, Chen: "Autophagy enhances the replication of classical swine fever virus in vitro." in: Autophagy, Vol. 10, Issue 1, pp. 93-110, 2013 (PubMed).

Polotskaia, Hoffman, Krett, Shanmugam, Rosen, Bargonetti: "8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers." in: Molecular cancer therapeutics, Vol. 11, Issue 11, pp. 2495-504, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Huang, Hou, Chen, Zhao, Yan, Zhang, Yang, Kogan, Chen: "PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway." in: Autophagy, Vol. 7, Issue 10, pp. 1132-44, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Neely, Green, LaFerla: "Presenilin is necessary for efficient proteolysis through the autophagy-lysosome system in a ?-secretase-independent manner." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 8, pp. 2781-91, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting