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VEGF antibody (Vascular Endothelial Growth Factor)

Details for Product anti-VEGF Antibody No. ABIN260967, Supplier: Log in to see
Antigen
  • VEGF
  • vegf-a
  • CG7103
  • DmVEGF-1
  • Dmel\\CG7103
  • EP1624
  • PVF
  • PVF1
  • PVFs
  • Pvf
  • VEGF2-B
  • Vegf17E
  • Vegf17e-a
  • Vegf17e-b
  • l(1)G0146
  • pvf1
  • vegf1
  • CG13780
  • DmVEGF-2
  • Dmel\\CG13780
  • PV2
  • PVF2
  • VEGF-2
  • VEGF27Cb
  • Vegf27Cb
  • pvf2
  • CG13781
  • CG13782
  • CG13783
  • CG31629
  • CG34378
  • CG4492
  • DmVEGF-3
  • Dmel\\CG34378
  • Dmel_CG13783
  • Dmel_CG31629
  • PVF3
  • VEGF27Ca
  • Vegf27Ca
  • pvf3
  • vegf
  • vascular endothelial growth factor A
  • vascular endothelial growth factor
  • PDGF- and VEGF-related factor 1
  • PDGF- and VEGF-related factor 2
  • PDGF- and VEGF-related factor 3
  • vascular endothelial growth factor precursor
  • vegfa-a
  • VEGF
  • Pvf1
  • Pvf2
  • Pvf3
  • vegf
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This VEGF antibody is un-conjugated
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Application
Cytometry by Time of Flight (CyTOF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
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Immunogen Recombinant VEGF 189 protein.
Clone VG1
Isotype IgG1 kappa
Specificity This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
Purification Protein G purified
Alternative Name VEGF
Background Gene Symbol: VEGFA
Gene ID 7422
UniProt P15692
Research Area Angiogenesis, Signaling, Growth Factors, Cancer, Organogenesis
Application Notes Western Blot 1:500, Flow Cytometry, ELISA, Immunohistochemistry 1:20-1:100, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:100, Immunohistochemistry-Frozen 1:20-1:100, CyTOF-readyThis VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1 mM EDTA, pH 8.0 must be performed prior to the immunostaining.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG1) [ABIN152088] - MCF7 cells...
Western Blotting (WB) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Western Blot: VEGF Antibody (VG1) [ABIN152088] - Western blot analysis of VEGF in hum...
Flow Cytometry (FACS) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Flow Cytometry: VEGF Antibody (VG1) [ABIN152088] - Analysis of PerCP conjugate of ABI...
Immunofluorescence (IF) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Immunocytochemistry/Immunofluorescence: VEGF Antibody (VG1) [ABIN152088] - HeLa cells...
Western Blotting (WB) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Western Blot: VEGF Antibody (VG1) [ABIN152088] - HUVEC and 293T Cells tested at 1:100...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-VEGF antibody (Vascular Endothelial Growth Factor) (ABIN260967) Immunohistochemistry-Paraffin: VEGF Antibody (VG1) [ABIN152088] - Analysis of Vascula...
Product cited in: Chen, Lin, Chen, Hsieh, Yen, Liang, Yang, Wang, Chen, Chow, Kao: "Immunohistochemical overexpression of hypoxia-induced factor 1α associated with slow reduction in (18)fluoro-2-deoxy-D-glucose uptake for chemoradiotherapy in patients with pharyngeal cancer." in: European journal of nuclear medicine and molecular imaging, Vol. 43, Issue 13, pp. 2343-2352, 2016 (PubMed). Further details: Immunohistochemistry

Su, Mar, Wu, Tai, Chu, Wu, Tseng, Lee, Chen, Liu, Chiu, Shiau: "Disrupting VEGF-A paracrine and autocrine loops by targeting SHP-1 suppresses triple negative breast cancer metastasis." in: Scientific reports, Vol. 6, pp. 28888, 2016 (PubMed). Further details: Immunohistochemistry

Chen, Jia, Kang, Zhang, Liu, Yang, Yan, Zuo, Guo, Jiang, Qi, Liu, Cui, Santos, Deng: "Upregulating Hif-1α by Hydrogel Nanofibrous Scaffolds for Rapidly Recruiting Angiogenesis Relative Cells in Diabetic Wound." in: Advanced healthcare materials, Vol. 5, Issue 8, pp. 907-18, 2016 (PubMed).

Lin, Chen, Chen, Hsieh, Yen, Liang, Yang, Wang, Chen, Chow, Kao: "Immunohistochemical studies and fluorodeoxyglucose uptake on positron emission tomography in pharyngeal cancer for predicting radiotherapy-based treatment outcomes." in: Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Ram, Singh, Kumawat, Kumar, Lingaraju, Uttam Singh, Rahal, Tandan, Kumar: "Deferoxamine modulates cytokines and growth factors to accelerate cutaneous wound healing in diabetic rats." in: European journal of pharmacology, Vol. 764, pp. 9-21, 2015 (PubMed). (Sample species: Rat (Rattus)).

Srinivas, Ramaiah, Lavanya, Yerramsetty, Kavi Kishor, Basha, Kamal, Bhadra, Bhadra: "Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142006, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Ram, Singh, Kumawat, Kant, Tandan, Kumar: "Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats." in: International immunopharmacology, Vol. 30, pp. 137-49, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Shao, Zhang, Yang, Qi, Zhang, Deng: "HIF-1α disturbs osteoblasts and osteoclasts coupling in bone remodeling by up-regulating OPG expression." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 8, pp. 808-14, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

André, Tunik, Aronsson, Kvanta: "Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model." in: Investigative ophthalmology & visual science, Vol. 56, Issue 11, pp. 6591-604, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Parlakgumus, Aka Bolat, Bulgan Kilicdag, Simsek, Parlakgumus: "Atorvastatin for ovarian torsion: effects on follicle counts, AMH, and VEGF expression." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 175, pp. 186-90, 2014 (PubMed). (Sample species: Rat (Rattus)).

Tam, Ko, Lau, To, Kwok, Chan, Siu, Etienne-Selloum, Lau, Chan, Leung, Fung, Schini-Kerth, Lau: "A Chinese 2-herb formula (NF3) promotes hindlimb ischemia-induced neovascularization and wound healing of diabetic rats." in: Journal of diabetes and its complications, Vol. 28, Issue 4, pp. 436-47, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Fedorova, Sodhi, Gatto-Weis, Puri, Hinds, Shapiro, Malhotra: "Peroxisome proliferator-activated receptor δ agonist, HPP593, prevents renal necrosis under chronic ischemia." in: PLoS ONE, Vol. 8, Issue 5, pp. e64436, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Johansen, Hasseldam, Rasmussen, Bisgaard, Bonfils, Poulsen, Hansen-Schwartz: "Drug-induced hypothermia as beneficial treatment before and after cerebral ischemia." in: Pathobiology : journal of immunopathology, molecular and cellular biology, Vol. 81, Issue 1, pp. 42-52, 2013 (PubMed).

Milanesi, Lee, Li, Da Sacco, Villani, Cervantes, Perin, Yu: "β-Cell regeneration mediated by human bone marrow mesenchymal stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42177, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Hong, Kurzrock, Oh, Wheler, Naing, Brail, Callies, André, Kadam, Nasir, Holzer, Meric-Bernstam, Fishman, Simon: "A phase 1 dose escalation, pharmacokinetic, and pharmacodynamic evaluation of eIF-4E antisense oligonucleotide LY2275796 in patients with advanced cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 20, pp. 6582-91, 2011 (PubMed).

Ptaszynska, Pendrak, Bandle, Stracke, Roberts: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Kunze, Wuttig, Kausch, Blietz, Blumhoff, Burmeister, Kraemer, Fuessel, Toma, Schwenzer, Meye, Grimm, Hakenberg, Jocham, Wirth: "Antisense-mediated inhibition of survivin, hTERT and VEGF in bladder cancer cells in vitro and in vivo." in: International journal of oncology, Vol. 32, Issue 5, pp. 1049-56, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Couvelard, OToole, Turley, Leek, Sauvanet, Degott, Ruszniewski, Belghiti, Harris, Gatter, Pezzella: "Microvascular density and hypoxia-inducible factor pathway in pancreatic endocrine tumours: negative correlation of microvascular density and VEGF expression with tumour progression." in: British journal of cancer, Vol. 92, Issue 1, pp. 94-101, 2005 (PubMed).

Van der Auwera, Van Laere, Van den Eynden, Benoy, van Dam, Colpaert, Fox, Turley, Harris, Van Marck, Vermeulen, Dirix: "Increased angiogenesis and lymphangiogenesis in inflammatory versus noninflammatory breast cancer by real-time reverse transcriptase-PCR gene expression quantification." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 23, pp. 7965-71, 2004 (PubMed).

Giatromanolaki, Koukourakis, Sivridis, Turley, Wykoff, Gatter, Harris: "DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer." in: The Journal of pathology, Vol. 200, Issue 2, pp. 222-8, 2003 (PubMed).