MHC Class II I-Ak antibody

Antigen: MHC Class II I-Ak

Clonality: Monoclonal (11-5-2)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN262496

Additional Information

Alternative name: MHC Class II (I-Ak)

Immunogen: CKB mouse splenocytes.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 11-5-2

Description: The 11-5.2 antibody reacts with the alpha chain of the I-Ak MHC class II alloantigen.These class II molecules are expressed on antigen presenting cells (including B cells)and a subset of T cells from H-2k bearing mice and are involved in antigen presentationto T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts withcells from mice bearing the H-2r haplotype. Reactivity with other H-2 haplotypes (e.g., b,d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to blockantigen presentation and induce B cell differentiation. Alternate Names: anti-MHC class II Ak antibody.

Specificity: Species Reactivity: Mouse k haplotype, Cross-reacts with H-2r haplotypes. Does not cross react with otherhaplotypes. (e.g., b, d, f, p, q, s).

Application Details

Application Notes: Positive Controls: B and activated T cells, APCs of H-2k mice

Buffer: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

C3H mouse splenocytes stained with 11-5.2 purified, followed by anti-mouse IgG2b FITC