MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (C-Term)

Details for Product anti-MELK Antibody No. ABIN263108, Supplier: Log in to see
Antigen
  • MELK
  • xmelk
  • Melk
  • HPK38
  • AI327312
  • MPK38
  • mKIAA0175
  • fb71b01
  • fe01f04
  • wu:fb71b01
  • wu:fe01f04
  • maternal embryonic leucine zipper kinase
  • MELK
  • melk
  • cgd5_2270
  • Melk
Epitope
C-Term
36
15
13
13
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
116
33
24
7
5
5
5
4
4
3
1
1
1
Host
Goat
94
18
10
Clonality
Polyclonal
Conjugate
This MELK antibody is un-conjugated
6
6
6
5
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
84
60
13
11
11
10
8
5
4
2
2
1
1
Options
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Immunogen Peptide with sequence KRLVEDILSSCKV corresponding to C-Terminus according to NP_055606.1.
Purification Immunogen affinity purified
Alternative Name MELK (MELK Antibody Abstract)
Background Gene Symbol: MELK
Gene ID 9833
NCBI Accession NP_055606
Research Area Embryogenesis, Embryonic stem cells, Hormones, Receptors
Application Notes Western Blot 0.1 - 0.3 μg/mL, Peptide ELISA Detection limit 1:32000WB: Experiments gave bands at approx. 75 kDa band and 150 kDa band in human testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The150 kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6 kDa band according to NP_055606.1.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (C-Term) (ABIN263108) Western Blot: MELK Antibody [ABIN263108] - ABIN263108 (0.1ug/ml) staining of HEPG2 ce...
Western Blotting (WB) image for anti-MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (C-Term) (ABIN263108) ABIN263108 (0.5µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). ...
Western Blotting (WB) image for anti-MELK antibody (Maternal Embryonic Leucine Zipper Kinase) (C-Term) (ABIN263108) Western Blot: MELK Antibody [ABIN263108] - MELK in human testes lysate (RIPA buffer, ...
Product cited in: Nagase, Seki, Ishikawa, Tanaka, Nomura: "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 1, pp. 17-24, 1996 (PubMed).