Maternal Embryonic Leucine Zipper Kinase (MELK) antibody

Antigen: Maternal Embryonic Leucine Zipper Kinase (MELK)

Synonyms: HPK38, KIAA0175, MPK38, AI327312, mKIAA0175, fb71b01, fe01f04, wu:fb71b01, wu:fe01f04, melk, MELK, Melk

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN263108

Additional Information

Alternative name: MELK

Gene ID: 9833

NCBI Acession: NP_055606

Immunogen: Synthetic peptide with sequence KRLVEDILSSCKV, representing the C-Terminus of theprotein (residues 639-651) according to NP_055606. Reference Sequence: NP_055606.

Cross-Reactivity: Human

Isotype: IgG

Description: MELK is a member of AMPK family, which has several consensus glycosylation sites.Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.Dand Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. OxfordUniversity Press]. Further the activity of this antibody was confirmed againstrecombinant, GST fused MELK protein by Western blotting. Alternate Names: anti-Likely ortholog of maternal embryonic leucine zipper kinase antibody, anti-Maternalembryonic leucine zipper kinase antibody.

Specificity: Species Reactivity: Cross-reacts with Human. Not yet tested in other species.

Application Details

Application Notes: Suggested working dilutions: Western Blot 0.5-2 ug/ml. Positive Controls: Jurkat and HeLa cell lysates

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris saline containing [pH7.3] with 0.5% BSA. Preservative: 0.02% Na azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Embryogenesis, Embryonic stem cells, Hormones, Receptors

Restrictions: For Research Use only

Images

Western Blot: MELK Antibody [ABIN263108] - ABIN263108 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue > NAC1 to Quaking > Goat Anti-PPP1R15A Antibody

ABIN263108 (0.5µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

Publications

Nagase, Seki, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 1, pp. 17-24, 1996 (PubMed).