Maternal Embryonic Leucine Zipper Kinase (MELK) antibody

Details for Product No. ABIN263108
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Antigen
Synonyms HPK38, MPK38, AI327312, mKIAA0175, fb71b01, fe01f04, wu:fb71b01, wu:fe01f04, xmelk, MELK, Melk
Reactivity
Human
(57), (20), (16), (15), (15), (12)
Host
Goat
(45), (10), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(47), (32), (12), (11), (10), (6), (5), (5), (3), (1)
Pubmed 1 reference available
Catalog no. ABIN263108
Quantity 0.1 mg
Price
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Immunogen Synthetic peptide with sequence KRLVEDILSSCKV, representing the C-Terminus of theprotein (residues 639-651) according to NP_055606. Reference Sequence: NP_055606.
Isotype IgG
Specificity Species Reactivity: Cross-reacts with Human. Not yet tested in other species.
Cross-Reactivity Human
Purification Aff - Purified
Alternative Name MELK
Background MELK is a member of AMPK family, which has several consensus glycosylation sites.Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.Dand Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. OxfordUniversity Press]. Further the activity of this antibody was confirmed againstrecombinant, GST fused MELK protein by Western blotting. Alternate Names: anti-Likely ortholog of maternal embryonic leucine zipper kinase antibody, anti-Maternalembryonic leucine zipper kinase antibody.
Gene ID 9833
NCBI Accession NP_055606
Research Area Embryogenesis, Embryonic stem cells, Hormones, Receptors
Application Notes Suggested working dilutions: Western Blot 0.5-2 ug/ml. Positive Controls: Jurkat and HeLa cell lysates
Restrictions For Research Use only
Concentration 0.5 mg/ml
Buffer Tris saline containing [pH7.3] with 0.5% BSA. Preservative: 0.02% Na azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibody Western Blot: MELK Antibody [ABIN263108] - ABIN263108 (0.1ug/ml) staining of HEPG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Online Catalogue > NAC1 to Quaking > Goat Anti-PPP1R15A Antibody
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibody (2) ABIN263108 (0.5µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
General Nagase, Seki, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell line KG-1." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 3, Issue 1, pp. 17-24, 1996 (PubMed).

Hosts (45), (10), (8)
Reactivities (57), (20), (16), (15), (15), (12)
Applications (47), (32), (12), (11), (10), (6), (5), (5), (3), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (13), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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