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CD83 antibody (CD83)

Details for Product anti-CD83 Antibody No. ABIN263553, Supplier: Log in to see
Antigen
  • BL11
  • HB15
Alternatives
anti-Human CD83 antibody for Flow Cytometry
Reactivity
Human
139
80
34
7
5
5
5
5
Host
Mouse
91
63
47
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD83 antibody is un-conjugated
29
25
12
7
6
6
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
121
49
31
27
22
20
11
9
5
5
2
2
2
2
1
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Immunogen COS cells transfected with the HB15 cDNA. Hybridization of NS1 myeloma cells with spleencells from Balb/c mice.
Clone HB15A
Isotype IgG2b
Specificity This antibody reacts to CD83.
Characteristics Synonyms: HB15, Cell surface protein HB15, B-cell activation protein
Purification Ion exchange or affinity chromatography
Alternative Name CD83 (CD83 Antibody Abstract)
Background The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.Synonyms: B-cell activation protein, Cell surface protein HB15, HB15
Gene ID 9308
UniProt Q01151
Research Area Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Fluorescent microscopy or flow cytometry, after dendritic cells enrichment by culture,enumeration and/or sorting of CD83+ cells. Studies of human dendritic cell subpopulations in tissues by immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL of distilled water
Buffer PBS containing 1 mg/mL BSA
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. e addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8 °C. Following reconstitution store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Product cited in: Ampem-Lassen, Sidiroglou, Peng et al.: "Index mapping for fibres with symmetric and asymmetric refractive index profiles." in: Optics express, Vol. 16, Issue 15, pp. 10912-7, 2008 (PubMed).

Fries, Heukeshoven, Hauber et al.: "Analysis of nucleocytoplasmic trafficking of the HuR ligand APRIL and its influence on CD83 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4504-15, 2007 (PubMed).

Prechtel, Chemnitz, Schirmer et al.: "Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 10912-25, 2006 (PubMed).

Background publications Zhou, Tedder: "Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3821-35, 1995 (PubMed).

Zhou, Tedder: "A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells." in: Blood, Vol. 86, Issue 9, pp. 3295-301, 1995 (PubMed).

Zhou, Schwarting, Smith et al.: "A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 735-42, 1992 (PubMed).