CD83 (CD83) antibody

Details for Product No. ABIN263553
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Antigen
Synonyms BL11, HB15
Reactivity
Human
(89), (55), (32), (5), (1)
Host
Mouse
(50), (41), (20), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(15), (14), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
(66), (36), (23), (20), (13), (8), (7), (3), (2), (1), (1)
Pubmed 6 references available
Catalog no. ABIN263553
Quantity 0.2 mg
Price
610.50 $   Plus shipping costs $45.00
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Immunogen COS cells transfected with the HB15 cDNA. Hybridization of NS1 myeloma cells with spleencells from Balb/c mice.
Clone HB15A
Isotype IgG2b
Characteristics Synonyms: HB15, Cell surface protein HB15, B-cell activation protein
Purification Ion exchange or affinity chromatography
Alternative Name CD83
Background The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a memberof the immunoglobulin superfamily and is composed of an extracellular V typeimmunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acidcytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as thedetermined Mr is twice the predicted size of the core protein. However, CD83+ cells have aunique cell surface immunophenotype that does not correlate with that of T cells, B cells,NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levelsof MHC class II molecules, when compared with other leucocyte lineages. They alsocoexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers(CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules.
UniProt Q01151
Research Area Immunology, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells
Application Notes Fluorescent microscopy or flow cytometry, after dendritic cells enrichment by culture,enumeration and/or sorting of CD83+ cells. Studies of human dendritic cell subpopulations in tissues by immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1mL of distilled water
Buffer PBS containing 1mg/ml BSA
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. e addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Product cited in: Prechtel, Chemnitz, Schirmer et al.: "Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 10912-25, 2006 (PubMed).

Fries, Heukeshoven, Hauber et al.: "Analysis of nucleocytoplasmic trafficking of the HuR ligand APRIL and its influence on CD83 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4504-15, 2007 (PubMed).

Ampem-Lassen, Sidiroglou, Peng et al.: "Index mapping for fibres with symmetric and asymmetric refractive index profiles." in: Optics express, Vol. 16, Issue 15, pp. 10912-7, 2008 (PubMed).

Background publications Zhou, Schwarting, Smith et al.: "A novel cell-surface molecule expressed by human interdigitating reticulum cells, Langerhans cells, and activated lymphocytes is a new member of the Ig superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 2, pp. 735-42, 1992 (PubMed).

Zhou, Tedder: "A distinct pattern of cytokine gene expression by human CD83+ blood dendritic cells." in: Blood, Vol. 86, Issue 9, pp. 3295-301, 1995 (PubMed).

Zhou, Tedder: "Human blood dendritic cells selectively express CD83, a member of the immunoglobulin superfamily." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3821-35, 1995 (PubMed).

Hosts (50), (41), (20), (2)
Reactivities (89), (55), (32), (5), (1)
Applications (66), (36), (23), (20), (13), (8), (7), (3), (2), (1), (1)
Conjugates (15), (14), (8), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (5), (3), (3), (2), (1), (1)
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