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Hsc70 antibody

HSPA8 Reactivity: Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Sheep, Rabbit, Pig, Guinea Pig, Drosophila melanogaster, Xenopus laevis, Saccharomyces cerevisiae, Fish, Beluga, Pinctada fucata (Pearl oyster), Scallop, Artemia sp. (Brine shrimp) WB, IP, IF, IHC (fro) Host: Mouse Monoclonal BB70 unconjugated
Catalog No. ABIN263941
  • Target See all Hsc70 (HSPA8) Antibodies
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Reactivity
    • 84
    • 46
    • 42
    • 21
    • 17
    • 14
    • 13
    • 11
    • 11
    • 10
    • 10
    • 10
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Cow, Chicken, Hamster, Dog, Sheep, Rabbit, Pig, Guinea Pig, Drosophila melanogaster, Xenopus laevis, Saccharomyces cerevisiae, Fish, Beluga, Pinctada fucata (Pearl oyster), Scallop, Artemia sp. (Brine shrimp)
    Host
    • 73
    • 27
    • 7
    • 2
    Mouse
    Clonality
    • 70
    • 38
    Monoclonal
    Conjugate
    • 61
    • 19
    • 11
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Hsc70 antibody is un-conjugated
    Application
    • 95
    • 53
    • 40
    • 32
    • 30
    • 30
    • 17
    • 15
    • 11
    • 11
    • 11
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Detects 72 and 73 kDa proteins corresponding to the Molecular Mass of inducible HSP and HSC70 on SDS PAGE Immunoblots.
    Characteristics
    Synonyms: HSP73, HSPA10, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8
    Purification
    Affinity Chromatography on Protein G
    Immunogen
    Chicken Hsp70/Hsp90 complex (1)
    Clone
    BB70
    Isotype
    IgG2a
    Top Product
    Discover our top product HSPA8 Primary Antibody
  • Application Notes
    Western blot (7): 1 μg/mL was sufficient for detection of hsp70/Hsc70 in 20 g of HeLalysate.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Vavilis, Delivanoglou, Aggelidou, Stamoula, Mellidis, Kaidoglou, Cheva, Pourzitaki, Chatzimeletiou, Lazou, Albani, Kritis: "Oxygen-Glucose Deprivation (OGD) Modulates the Unfolded Protein Response (UPR) and Inflicts Autophagy in a PC12 Hypoxia Cell Line Model." in: Cellular and molecular neurobiology, Vol. 36, Issue 5, pp. 701-12, (2016) (PubMed).

    Stamoula, Vavilis, Aggelidou, Kaidoglou, Cheva, Mellidis, Lazou, Haitoglou, Albani, Kritis: "Low Dose Administration of Glutamate Triggers a Non-Apoptotic, Autophagic Response in PC12 Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 37, Issue 5, pp. 1750-8, (2015) (PubMed).

    Taldone, Kang, Patel, Patel, Patel, Rodina, Patel, Gozman, Maharaj, Clement, Lu, Young, Chiosis et al.: "Heat shock protein 70 inhibitors. 2. 2,5'-thiodipyrimidines, 5-(phenylthio)pyrimidines, 2-(pyridin-3-ylthio)pyrimidines, and 3-(phenylthio)pyridines as reversible binders to an allosteric site on ..." in: Journal of medicinal chemistry, Vol. 57, Issue 4, pp. 1208-24, (2014) (PubMed).

    Rodina, Taldone, Kang, Patel, Koren, Yan, DaGama Gomes, Yang, Patel, Shrestha, Ochiana, Santarossa, Maharaj, Gozman, Cox, Erdjument-Bromage, Hendrickson, Cerchietti, Melnick, Guzman, Chiosis: "Affinity purification probes of potential use to investigate the endogenous Hsp70 interactome in cancer." in: ACS chemical biology, Vol. 9, Issue 8, pp. 1698-705, (2014) (PubMed).

    Rodina, Patel, Kang, Patel, Baaklini, Wong, Taldone, Yan, Yang, Maharaj, Gozman, Patel, Patel, Chirico, Erdjument-Bromage, Talele, Young, Chiosis: "Identification of an allosteric pocket on human hsp70 reveals a mode of inhibition of this therapeutically important protein." in: Chemistry & biology, Vol. 20, Issue 12, pp. 1469-80, (2013) (PubMed).

    Guess, Ayoob, Chanley, Manley, Cajaiba, Agrawal, Pengal, Pyle, Becknell, Kopp, Ronkina, Gaestel, Benndorf, Smoyer: "Crucial roles of the protein kinases MK2 and MK3 in a mouse model of glomerulonephritis." in: PLoS ONE, Vol. 8, Issue 1, pp. e54239, (2013) (PubMed).

  • Target
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Alternative Name
    HSPA8 / HSC70 (HSPA8 Products)
    Synonyms
    hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody
    Background
    Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.Synonyms: HSP73, HSPA10, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein
    Gene ID
    3312
    UniProt
    P11142
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