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CD74 antibody (HLA-DR-gamma)

Details for Product anti-CD74 Antibody No. ABIN263955, Supplier: Log in to see
Antigen
  • CLIP
  • DHLAG
  • HLADG
  • Ia-GAMMA
  • Ii
  • II
  • INVG34
Reactivity
Human
401
101
25
24
1
1
1
Host
Mouse
363
50
10
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is un-conjugated
29
23
21
13
13
12
11
10
10
8
8
8
8
8
8
8
7
7
6
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
318
217
195
145
130
115
107
84
59
11
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Immunogen Human CD74 invariant chain synthetic peptide conjugated to KLH
Clone PIN-1
Isotype IgG1
Specificity Detects ~33-35 kDa protein doublet, corresponding to the molecular mass of the p33 and p35 forms of human CD74 on SDS-PAGE immunoblots.
Characteristics Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain
Purification Purified
Alternative Name CD74 (CD74 Antibody Abstract)
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC), however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).Synonyms: DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33
Gene ID 972
UniProt P04233
Research Area Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Application Notes Western blot (1 μg/mL was sufficient for detection using colorimetric western blot analysis). Immunoprecipitation(5). Immunoflourescence(6). Flow cytometry. FACS (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2, 0.09 % azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Starlets, Gore, Binsky, Haran, Harpaz, Shvidel, Becker-Herman, Berrebi, Shachar: "Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival." in: Blood, Vol. 107, Issue 12, pp. 4807-16, 2006

Becker-Herman, Arie, Medvedovsky, Kerem, Shachar: "CD74 is a member of the regulated intramembrane proteolysis-processed protein family." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5061-9, 2005

Barrera, Beswick, Sierra, Bland, Espejo, Mifflin, Adegboyega, Crowe, Ernst, Reyes: "Polarized expression of CD74 by gastric epithelial cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 53, Issue 12, pp. 1481-9, 2005

Burton, Ely, Reddy, Stein, Gold, Cardillo, Goldenberg: "CD74 is expressed by multiple myeloma and is a promising target for therapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6606-11, 2004

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, 1997

Denzin, Robbins, Carboy-Newcomb, Cresswell: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995