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Details for Product No. ABIN263955

HLA-DR-gamma (CD74) antibody

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Antigen
Synonyms DHLAG, HLADG, II, Ia-GAMMA
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 6 references available
Catalog no. ABIN263955
Quantity 25 µg
Price
187.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Human CD74 invariant chain synthetic peptide conjugated to KLH
Clone PIN-1
Isotype IgG1
Characteristics Synonyms: p33, DHLAG, HLA class II histocompatibility antigen gamma chain, HLA-DRantigens-associated invariant chain, Ia antigen-associated invariant chain
Purification Purified
Alternative Name CD74
Background CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminalcytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region andan approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainlyas an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directsthem to endocytic compartments, prevents peptide binding in the ER, and contributes topeptide editing in the MHC class II compartment. Class II MHC and Ii expression wasbelieved to be restricted to classical antigen-presenting cells (APC), however, duringinflammation, other cell types, including mucosal epithelial cells, have also been reportedto express class II MHC molecules (2). Experiments that investigate cell-surface CD74 arecomplicated by the fact that CD74 remains on the cell surface for a very short time. Thesurface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however hasalso recently been shown to have a role as an accessory-signaling molecule because of itshigh-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitoryfactor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapidinternalization make CD74 an attractive therapeutic target for both cancer andimmunologic diseases (4).
UniProt P04233
Research Area Immunology, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Major Histocompatibility Complex (MHC)
Application Notes Western blot (1 ug/ml was sufficient for detection using colorimetric western blot analysis). Immunoprecipitation(5). Immunoflourescence(6). Flow cytometry. FACS (6). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2, 0.09 % azide and 50 % glycerol
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Denzin, Robbins, Carboy-Newcomb et al.: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, 1995 (PubMed).

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, 1997 (PubMed).

Burton, Ely, Reddy et al.: "CD74 is expressed by multiple myeloma and is a promising target for therapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6606-11, 2004 (PubMed).

Barrera, Beswick, Sierra et al.: "Polarized expression of CD74 by gastric epithelial cells." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 53, Issue 12, pp. 1481-9, 2005 (PubMed).

Becker-Herman, Arie, Medvedovsky et al.: "CD74 is a member of the regulated intramembrane proteolysis-processed protein family." in: Molecular biology of the cell, Vol. 16, Issue 11, pp. 5061-9, 2005 (PubMed).

Starlets, Gore, Binsky et al.: "Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival." in: Blood, Vol. 107, Issue 12, pp. 4807-16, 2006 (PubMed).

Alternatives for antigen "HLA-DR-gamma (CD74)", type "Antibodies"
Hosts (136), (26), (9)
Reactivities (162), (20), (18), (17), (17), (17), (3), (3), (2), (1), (1), (1), (1), (1)
Applications (125), (68), (63), (51), (14), (13), (13), (12), (11), (10), (6), (5), (2), (2), (1), (1), (1), (1)
Conjugates (17), (12), (11), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (2), (1), (1), (1)
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