Heat Shock Factor 1 (HSF1) antibody

Details for Product No. ABIN263956
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Synonyms AA960185
Cow (Bovine), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(27), (10), (5), (3), (3), (2), (2), (2), (1)
(18), (7), (5)
Clonality (Clone)
Monoclonal ()
ELISA, Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(30), (15), (11), (8), (8), (7), (2), (2), (1)
Pubmed 10 references available
Quantity 25 μg
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Catalog No. ABIN263956
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Immunogen Purified recombinant HSF1 protein
Clone 10H8
Isotype IgG1
Characteristics Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1
Purification Purified
Alternative Name Heat shock factor 1 / HSF1
Background HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors thatactivate the transcription of genes encoding products required for protein folding,processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shockproteins) expression by stressors is achieved at the level of transcription through a heatshock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highlyconserved amino acid sequences. On all HSFs there is a DNA binding domain at theNterminus. Hydrophobic repeats located adjacent to this binding domain are essential forthe formation of active trimers. Towards the C-terminal region another short hydrophobicrepeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms,however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nucleardistribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes todiscrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates fromthese granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar tomouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally activethan the smaller beta form (7, 8). Various experiments have suggested that HFS2 may haveroles in differentiation and development (9, 10, 11).
UniProt Q00613
Application Notes Western blot (1): 1 ug/ml was sufficient for detection of HSF1 by ECL immunoblot in 20 g ofHeLa lysate. Immunoprecipitation. Immunflourescence (1). ELISA (1). Gel Mobility Shift Assay (1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % sodium azide in 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Goodson, Park-Sarge, Sarge: "Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities." in: Molecular and cellular biology, Vol. 15, Issue 10, pp. 5288-93, 1995 (PubMed).

Fiorenza, Farkas, Dissing et al.: "Complex expression of murine heat shock transcription factors." in: Nucleic acids research, Vol. 23, Issue 3, pp. 467-74, 1995 (PubMed).

Murphy, Gorzowski, Sarge et al.: "Characterization of constitutive HSF2 DNA-binding activity in mouse embryonal carcinoma cells." in: Molecular and cellular biology, Vol. 14, Issue 8, pp. 5309-17, 1994 (PubMed).

Sarge, Park-Sarge, Kirby et al.: "Expression of heat shock factor 2 in mouse testis: potential role as a regulator of heat-shock protein gene expression during spermatogenesis." in: Biology of reproduction, Vol. 50, Issue 6, pp. 1334-43, 1994 (PubMed).

Rallu, Loones, Lallemand et al.: "Function and regulation of heat shock factor 2 during mouse embryogenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2392-7, 1997 (PubMed).

Cotto, Fox, Morimoto: "HSF1 granules: a novel stress-induced nuclear compartment of human cells." in: Journal of cell science, Vol. 110 ( Pt 23), pp. 2925-34, 1998 (PubMed).

McMillan, Xiao, Shao et al.: "Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7523-8, 1998 (PubMed).

Morimoto: "Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators." in: Genes & development, Vol. 12, Issue 24, pp. 3788-96, 1999 (PubMed).

Jolly, Usson, Morimoto: "Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6769-74, 1999 (PubMed).

Morano, Thiele: "Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signals." in: Gene expression, Vol. 7, Issue 4-6, pp. 271-82, 1999 (PubMed).

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