Lysosomal-Associated Membrane Protein 2 (LAMP2) antibody

Details for Product No. ABIN263966
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Antigen
Synonyms cd107b, lamp-2, lampb, LAMP2, lamp2, DKFZp459J126, LAMP-2, LGP-B, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3, LAMPB, LGP110
Reactivity
Mouse (Murine), Rabbit
(125), (118), (24), (21), (13), (13), (6), (2)
Host
Rat
(89), (85), (54), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(32), (22), (15), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(102), (100), (59), (53), (48), (41), (30), (30), (28), (10), (2), (2)
Pubmed 6 references available
Quantity 25 μg
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Catalog No. ABIN263966
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Immunogen Purified preparation of mouse liver lysosomal membranes
Clone GL2A7
Isotype IgG1
Characteristics Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Purification Protein G chromatography
Alternative Name CD107b / LAMP2
Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents ofthe lysosomal membrane. The two have closely related structures, with 37% sequencehomology (2). They are both transmembrane glycoproteins that are localized primarily inlysosomes and late endosomes. Newly synthesized molecules are mostly transported fromthe trans-Golgi network directly to endosomes and then to lysosomes. A second pathwayinvolves the lamps being delivered from the Golgi to the cell surface, and then along theendocytic pathway to the lysosomes. A minor pathway involves transport via the plasmamembrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells thatsecrete lysosomal hydrolases. A study in the developmental expresses patterns ofmembrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell orcellextracellular matrix interaction, and appear to reflect tissue and cell type specific rolesof lysosomes duringmorphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane isdependent on a carboxylterminal tyrosine based motif (YXXI) (5). This stimulation has alsobeen shown to have an associated release of histamine, leukotriene C 9$) andprostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers fornormal mast cells (5). They have also been linked to the inflammatory response in that theypromote adhesion of human peripheral blood mononuclear cells (PBMC) to vascularendothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagicvacuoles to vacuoles which rapidly degrade their content (7).
UniProt P17047
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Immunoprecipitation (1). Immunoflourescence: 1: 500-1: 1000 (1).
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS pH 7.4, 0.09 % azide, in 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Granger, Green, Gabel et al.: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Lichter-Konecki, Moter, Krawisz et al.: "Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis." in: Differentiation; research in biological diversity, Vol. 65, Issue 1, pp. 43-58, 1999 (PubMed).

Tanaka, Guhde, Suter et al.: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

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