Perforin 1 (Pore Forming Protein) (PRF1) antibody

Details for Product No. ABIN264014
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(127), (54), (31), (3), (1), (1)
(75), (73), (22)
Clonality (Clone)
Monoclonal ()
(16), (9), (8), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Flow Cytometry (FACS)
(92), (52), (42), (35), (28), (24), (23), (18), (17), (11), (10), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 8 references available
Quantity 0.1 mg
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Catalog No. ABIN264014
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Clone 4E4
Isotype IgG2a
Characteristics Synonyms: Perforin-1, Pore forming protein, PFN, PFP, Cytolysin, Lymphocyte pore-forming protein
Purification Purified
Alternative Name Perforin 1 / PRF1
Background Perforin is one of the major cytolytic proteins of cytolytic granules. One of the mainpathways of lymphocyte mediated cytolysis entails the secretion onto target membranes ofcytolytic granules contained in cytolytic effector lymphocytes of T cell or NK cell type. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Perforin is involved in the killing mediated by cytotoxic lymphocytes, which represents animportant mechanism in the immune defense against tumors and virus infections. Humanperforin is a 555 amino acid protein with a 21 amino acid signal peptide. It has a molecularweight of 70 to 75 kDa. Perforin is a pore forming protein with a mechanism oftransmembrane channel formation similar to C9 and homology between perforin and C9has been demonstrated at their respective functionally conserved regions. Studies showthat perforin is expressed only in killer cell lines and not in helper T lymphocytes or othertumor cells tested.
UniProt P14222
Application Notes Flow cytometry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5mg/mL (OD280nm)
Buffer 100mM Borate buffered saline, pH 8.2
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Gupta, Greer, Mahanty et al.: "CD8-mediated protection against Ebola virus infection is perforin dependent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 7, pp. 4198-202, 2005 (PubMed).

Rode, Balkow, Sobek et al.: "Perforin and Fas act together in the induction of apoptosis, and both are critical in the clearance of lymphocytic choriomeningitis virus infection." in: Journal of virology, Vol. 78, Issue 22, pp. 12395-405, 2004 (PubMed).

Smyth, Thia, Cretney et al.: "Perforin is a major contributor to NK cell control of tumor metastasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 11, pp. 6658-62, 1999 (PubMed).

Braun, Lowin, French et al.: "Cytotoxic T cells deficient in both functional fas ligand and perforin show residual cytolytic activity yet lose their capacity to induce lethal acute graft-versus-host disease." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 657-61, 1996 (PubMed).

Lowin, Hahne, Mattmann et al.: "Cytolytic T-cell cytotoxicity is mediated through perforin and Fas lytic pathways." in: Nature, Vol. 370, Issue 6491, pp. 650-2, 1994 (PubMed).

Masson, Tschopp: "Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes." in: The Journal of biological chemistry, Vol. 260, Issue 16, pp. 9069-72, 1985 (PubMed).

Lichtenheld, Olsen, Lu et al.: "Structure and function of human perforin." in: Nature, Vol. 335, Issue 6189, pp. 448-51, 1988 (PubMed).

Hameed, Olsen, Cheng et al.: "Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody." in: The American journal of pathology, Vol. 140, Issue 5, pp. 1025-30, 1992 (PubMed).

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