T Cell Receptor (TCR) Va 24 antibody (Biotin)

Antigen: T Cell Receptor (TCR) Va 24

Clonality: Monoclonal (C15)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN264099

Additional Information

Characteristics: Synonyms: TCR V alpha-24

Immunogen: Human T-Cell clone

Cross-Reactivity: Human

Isotype: IgG1

Clone: C15

Description: A population of mature CD4 CD8 double-negative T cells that expresses an invariant Valpha 24-J alpha Q TCR has been identified in humans, the majority of these cells appear toexpress V beta 11. A closely related invariant TCR alpha chain is also expressed by apopulation of NK1+ murine T cells, but these cells may be either CD4+ or double negative.

Specificity: This antibody reacts to variable alpha-24 chain of the T cell receptor also called TCRAV24S1according to the nomenclature from Arden et al. V-alpha-24 is a single memberedsubfamily (IGRa02 sequence). The antibody has been described in, it was characterized onhuman T cell clones and by extensive cell sorting followed by molecular biology analysis. V-alpha-24 has been shown to be expanded in a clonal fashion in the CD3+ CD8- CD4- cellpopulation in PBL of several donors. The specificity of this antibody has been studied atthe First Human TcR Monoclonal Antibody Workshop in San Francisco in 1995. Species: Human. Others not tested.

Application Details

Application Notes: T-cell repertoire studiesFluorescent microscopy or flow cytometry: 2g/5x10e5 cells/tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 1mg/ml BSALabel: BiotinReconstitution: Restore with 0.5ml distilled water

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only