T Cell Receptor (TCR) Va 24 antibody (FITC)

Antigen: T Cell Receptor (TCR) Va 24

Clonality: Monoclonal (C15)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN264100

Additional Information

Characteristics: Synonyms: TCR V alpha-24, TCR Va24

Immunogen: Human T-cell clone. Mouse. Hybridization of PAI myeloma cells with spleen cells from Balb/c mice.

Cross-Reactivity: Human

Isotype: IgG1

Clone: C15

Description: A population of mature CD4 CD8 double-negative T cells that expresses an invariant Valpha 24-J alpha Q TCR has been identified in humans, the majority of these cells appear toexpress V beta 11. A closely related invariant TCR alpha chain is also expressed by apopulation of NK1+ murine T cells, but these cells may be either CD4+ or double negative.

Specificity: Human variable alpha-24 chain of the T cell receptor also called TCRAV24S1 according tothe nomenclature from Arden et al. (1). V-alpha-24 is a single membered subfamily (IGRa02sequence (2)). The antibody has been described in (3,4), it was characterized on human Tcell clones and by extensive cell sorting followed by molecular biology analysis. V-alpha-24 has been shown to be expanded in a clonal fashion in the CD3+ CD8- CD4- cellpopulation in PBL of several donors. (3,4). The specificity of this antibody has been confirmed at the First Human TcR MonoclonalAntibody Workshop in San Francisco in 1995 (Ref. 5). Species: Human. Others not tested.

Application Details

Application Notes: T-cell repertoire research studies. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 25 µg/ml

Buffer: PBS containing 2 mg/ml BSA as stabilizer and 0.09% Sodium Azide aspreserative.Label: FITC

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only