T Cell Receptor (TCR) Vb 11 antibody

Antigen: T Cell Receptor (TCR) Vb 11

Clonality: Monoclonal (C21)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN264102

Additional Information

Characteristics: Synonyms: TCR V beta-11, TCR Vb11

Immunogen: Human T-Cell clone

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: C21

Description: T cell receptors (TCR) recognize foreign antigens which have been processed as smallpeptides and bound to major histocompatibility complex (MHC) molecules at the surfaceof antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alphaand one beta chain or one delta and one gamma chain. This region represents the germlineorganization of the T cell receptor beta locus. The beta locus includes V (variable), J(joining), diversity (D), and C (constant) segments. During T cell development, the betachain is synthesized by a recombination event at the DNA level joining a D segment with a Jsegment, a V segment is then joined to the D-J gene. The C segment is later joined bysplicing at the RNA level.

Specificity: This antibody reacts to the variable beta-11 chain of the T cell receptor also calledTCRBV11S1 according to the nomenclature from Wei et al. Two V beta-11 sequences havebeen described, PL3. 12 and PH15. These sequences differ only in their leader sequence andtherefore lead to the same mature protein. C21 recognizes the gene product of thesesequences. It has been characterized on human T cell clones and by extensive cell sortingfollowed by molecular biology analysis. V beta-11 has been shown to be expanded in aclonal fashion in the CD3+ CD8- CD4- cell population in PBL of several donors. Species: Human. Others not tested.

Application Details

Application Notes: T-cell repertoire studies. Flow cytometry: 2g/5x10e5 cells/tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: PBS containing 1mg/ml BSAReconstitution: Restore with 0.5ml of distilled water

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to 1 month at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Immunology, Receptors

Restrictions: For Research Use only