T Cell Receptor (TCR) Vb 17 antibody

Antigen: T Cell Receptor (TCR) Vb 17

Clonality: Monoclonal (E17-5F3)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN264105

Additional Information

Characteristics: Synonyms: TCR V beta-17, TCR Vb17

Immunogen: Murine T-cell hybridoma transfected with V beta-17 gene segment.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: E17-5F3

Specificity: This antibody reacts to the variable beta-17 chain of the T-cell receptor also calledTCRBV17S1 according to the nomenclature of Wei et al. . V beta-17 is a single memberedfamily (HBVT02). This antibody has been further characterized by cell sorting on PBL usingthis antibody followed by analysis of sorted cells by molecular biology. Analysis of alphachain mRNA by PCR with a panel of a specific oligonucleotides shows transcripts for most Valpha sequences. Analysis of beta chain mRNA by anchored PCR and sequencing, onlyshows transcripts for beta-17 gene segment (HBVT02 sequence). Species: Human. Others not tested.

Application Details

Application Notes: Flow cytometry: 2g/5x10e5 cells/test. Studies have shown that V beta-17 may be useful in T-cell repertoire studies in normal andpathological situations including autoimmune disease, particularly rheumatoid arthritisand AIDS. Superantigenic stimulation of T cells, V beta-17 seems to be the target of MAM(Mycoplasma Arthritis derived superantigen). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2mg/ml (prior to lyophilization)

Purification: Purified

Buffer: PBS containing 1mg/ml BSAReconstitution: Restore with 0.5ml distilled water.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. The addition of 0. 1% (w/v) sodium azide recommended for storage up to one month at 2 -8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only