T Cell Receptor (TCR) Vb 5.2 antibody (PE)

Antigen: T Cell Receptor (TCR) Vb 5.2

Clonality: Monoclonal (36213)

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN264111

Additional Information

Characteristics: Synonyms: TCR V beta-5.2, TCR Vb5.2

Immunogen: 1C1 V beta 5. 2 positive cell line.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 36213

Specificity: This antibody reacts to variable beta 5. 2 chain of the T-cell receptor also called TCRBV5S2according to the nomenclature from Wei et al. . This antibody recognizes only the PL2. 5sequence. It does not recognize the V beta 5. 1 [HPB51 sequence and the V beta 5. 3 (IGRb08sequence]. Recognition of other members of the V beta 5 family has not been detected butcannot be formally excluded. Cell sorting on PBL gives a heterogenous pattern of stainingof the sorted cells. The antibody may thus be sensitive to the clonotypic environment (Valpha, J alpha, D beta, J beta) though molecular analysis of transcripts from the sorted Vbeta 5. 2 positive cells shows defferent V alpha, D and J beta. The specificity of thisantibody has been confirmed at the First Human TcR Monoclonal Antibody Workshop inSan Francisco in 1995. Species: Human. Others not tested.

Application Details

Application Notes: T-cell repertoire studies. Flow cytometry: 20l/5 x 10e5 cells/test or 100l whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 12.5 µg/ml

Buffer: PBS containing 2mg/ml BSA and 0,09% sodium azideLabel: Phycoerythrin

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only