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CD3 antibody (APC)

CD3 Reactivity: Human FACS Host: Mouse Monoclonal M2AB APC

Catalog No. ABIN264182

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
189

90

23

15

15

10

8

8

7

5

3

3

2

1
Human
Host
215

45

14

13

9
Mouse
Compatible Secondaries
Clonality
285

7
Monoclonal
Conjugate
88

33

29

23

22

11

7

7

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to APC
Application
267

81

55

44

43

40

28

23

9

9

8

7

4

3

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS)

Characteristics

Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Clone

M2AB

Isotype

IgG1

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anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

Flow Cytometry. This antibody is suitable for Monitoring of T cells subsets in peripheral blood,Characterization of subtypes of T cell leukemia's and lymphomas, Studies of AIDS/HIVvirus infection, Analysis of CD3 complex related to the T cell antigen receptor.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol

PBMC: Add 10 ul of Antibody/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.

Restrictions

For Research Use only
Buffer

PBS with 0.08 % sodium azide as preservative and 0.2 % protein carrier.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C

Storage Comment

Store the antibody (undiluted) at 2-8 °C. Do Not Freeze!
Shelf life: One year from despatch.

Expiry Date

12 months
Target

CD3

Alternative Name

CD3 (CD3 Products)

Synonyms

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background

The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85 % of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M.W. surface antigen.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID

916

UniProt

P07766

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway