You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD11b antibody (Integrin alpha M) (PE)

Details for Product anti-ITGAM Antibody No. ABIN264315, Supplier: Login to see
Antigen
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
Alternatives
anti-Human CD11b antibody for Immunoprecipitation
Reactivity
Human
558
439
133
45
20
17
17
17
17
11
10
9
5
4
3
2
2
1
Host
Mouse
455
397
106
17
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD11b antibody is conjugated to PE
165
121
87
49
18
17
12
11
11
8
7
7
7
7
6
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
769
215
178
136
117
84
78
61
48
35
19
16
10
7
6
5
4
3
3
3
3
3
2
1
1
1
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Clone F6-2
Isotype IgG2a
Characteristics Synonyms: Integrin alpha-M, CR3A, MAC-1, MAC1, CR-3 alpha chain, Cell surface glycoprotein MAC-1subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11antigen-like family member B
Alternative Name CD11b (ITGAM Antibody Abstract)
Background (Mac-1), CD11b/CD18 complex is a leukocyte adhesion receptor to intercellular adhesion molecules (ICAM's). CR3 alpha chain (M.W. 165,000) is on virtually all peripheral blood neutrophils, eosinophils, monocytes and approximately 30 % of peripheral blood lymphocytes.Synonyms: CD11 antigen-like family member B, CR-3 alpha chain, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, ITGAM, Integrin alpha-M, Leukocyte adhesion receptor MO1, MAC1, Neutrophil adherence receptor
Gene ID 3684
UniProt P11215
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Glia marker, CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis
Application Notes Flow Cytometry. Monitoring of subsets in peripheral blood. Characterization of leukemias and lymphomas. Analysis of CTL-mediated cytotoxicity. Studies of transplantation rejection. Functional studies of cell adhesion molecules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol PBMC: Add 10 ul of MAB/10e6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0. 5% v/v of paraformaldehyde in PBS andanalyze.
Restrictions For Research Use only
Buffer Sterile filtered PBS containing 0.2 % protein carrier and 0.08 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-CD11b antibody (Integrin alpha M)  (PE) (ABIN264315) anti-Integrin alpha M (ITGAM) antibody (PE)
Background publications Anderson, Springer: "Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins." in: Annual review of medicine, Vol. 38, pp. 175-94, 1987 (PubMed).

Richter, Ng-Sikorski, Olsson et al.: "Tumor necrosis factor-induced degranulation in adherent human neutrophils is dependent on CD11b/CD18-integrin-triggered oscillations of cytosolic free Ca2+." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 23, pp. 9472-6, 1991 (PubMed).

Merrill, Slade, Weissmann et al.: "Two pathways of CD11b/CD18-mediated neutrophil aggregation with different involvement of protein kinase C-dependent phosphorylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 8, pp. 2608-15, 1990 (PubMed).

Arnaout: "Structure and function of the leukocyte adhesion molecules CD11/CD18." in: Blood, Vol. 75, Issue 5, pp. 1037-50, 1990 (PubMed).

Babcock, Alexander, Warden: "Flow cytometric analysis of neutrophil subsets in thermally injured patients developing infection." in: Clinical immunology and immunopathology, Vol. 54, Issue 1, pp. 117-25, 1990 (PubMed).