Nerve Growth Factor (NGF) antibody (Biotin)

Antigen: Nerve Growth Factor (NGF)

Synonyms: NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428, Ngfb, ngfb, ns:zft386, xx:zft386, zgc:109730, NGF, beta-NGF, hsan5, beta-ngf

Clonality: Monoclonal (AS18)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA)

Catalog no.: ABIN264352

Additional Information

Characteristics: Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor

Alternative name: NGF

Swiss-Prot: P01138

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine)

Isotype: IgG1

Clone: AS18

Description: Nerve growth factor (NGF) influences the survival and differentiation of a variety ofneuronal and nonneuronal cells. The actions of this polypeptide result from binding tospecific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signaltransduction. Cross-linking studies with 125I-NGF have shown that the high affinityreceptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinityreceptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and ratNGF receptor genes have been produced and sequenced, revealing greater than 90%homology between the two species. Recently, it has been shown that cells which do notnormally respond to NGF only express low affinity receptors from transfected NGF genesbut cells which normally are NGF-responsive express high affinity receptors from the sameNGF sequences. This supports the hypothesis that the high affinity receptors have a uniquecomponent in addition to the subunit common to the low affinity receptors.

Specificity: Recognizes human, bovine, viper and mouse 2. 5S, 7S and bNGF. The epitope has beenlocalized to the C-terminus of hNGF. Species: Human, Mouse, Bovine and Viper.

Application Details

Application Notes: Elisa: This antibody has been used as a solution phase antibody at 2 ug/ml in a two siteELISA with unconjugated NGF antibody (ABIN264354 or ABIN264355) for mouse NGFand AS22 for human NGF. Typically for human NGF, the ELISA has a working range of 0-15ng/ml and a sensitivity of 15 pg/ml with the appropriate standard. Recommended samplevolumes are 25-200 l. Recovery of human NGF in human plasma is typically > 90% in theELISA. The use of Tris buffer, pH 7, instead of PBS is recommended. (Kenney, JS, Masada,MP, and Allison AC, Laboratory Methods in Immunology, Vol. I, 231, 1990). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: Lot specific

Buffer: PBS containing 0.08% Sodium Azide as preservative.Label: Biotin

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Neurology, Growth Factors

Restrictions: For Research Use only