CAPN1 antibody
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- Target See all CAPN1 Antibodies
- CAPN1 (Calpain 1 (CAPN1))
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Reactivity
- Human, Mouse, Rat, Cow
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CAPN1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody (large subunit, p80) recognizes the 80 kDa subunit of μ-Calpain as well as two smaller proteins that are presumed to be autolysis products.
- Characteristics
- Synonyms: Calpain mu-type, muCANP, CAPN1, CANPL1, PIG30, CANP1, Calpain-1 catalytic subunit,Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Micromolar-calpain, Cellproliferation-inducing gene 30 protein
- Purification
- Standard chromatographic techniques.
- Immunogen
- Calpain purified from human placenta. Genename: CAPN1
- Clone
- 15C10
- Isotype
- IgG1
- Top Product
- Discover our top product CAPN1 Primary Antibody
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- Application Notes
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ELISA: 0.5-1 μg/mL. Western blot: 0.5-1 μg/mL. Immunoprecipitation of both the native and denatured protein: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Buffer
- 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50 % Glycerol and 3 mM Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. - Expiry Date
- 12 months
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- Target
- CAPN1 (Calpain 1 (CAPN1))
- Alternative Name
- Calpain-1 (CAPN1 Products)
- Synonyms
- CAPN1 antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, Capn1 antibody, fj67g01 antibody, wu:fj67g01 antibody, calpain 1 antibody, zgc:55262 antibody, CAPN1 antibody, Capn1 antibody, zgc:55262 antibody
- Background
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and -calpain (calpain I) are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca ++ requirements that are suggested by their names. Whereas m-calpain requires millimolar ( mM) levels of calcium, μ-calpain is active at micromolar (μM) concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.Synonyms: CANP1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, PIG30, muCANP
- Gene ID
- 823
- UniProt
- P07384
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