T Cell Factor 3/4 (TCF3/4) antibody

Antigen: T Cell Factor 3/4 (TCF3/4)

Clonality: Monoclonal (6F12-3)

Host: Mouse

Reactivity: Human

Application: Electrophoretic Mobility-Shift Assay (EMSA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN264394

Additional Information

Characteristics: Synonyms: T-Cell Factor-3/4, TCF-3, TCF-4

Immunogen: His-tagged fusion protein corresponding to amino acids 31-331 of the human TCF.

Cross-Reactivity: Human, Mouse (Murine)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6F12-3

Description: TCF-3 and TCF-4 (T cell factor-4) are a members of the TCF/LEF (lymphoid enhancer factor)family of transcription factors. Members of this family along with catenin, are involved inmediating Wnt signaling by transactivating downstream genes. TCF-4 generatestranscriptionally active complexes with catenin in colon carcinoma cells. Expression ofTCF-4 is highest in the CNS and intestinal epithelium where Wnt signaling also plays anessential role in development. Aberrant TCF-4 transcription activity has been implicated incarcinogenesis of intestinal and mammary epithlia. TCF-3 appears to be expressedexclusively of TCF-4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.

Specificity: Species: Human and Mouse. Others not tested.

Application Details

Application Notes: Western Blotting: Detects TCF-4 at 1-4 ug/ml and a 60 kDa band in Cos-1 cells transfectedwith human TCF-3. Immunohistochemistry (Paraffin Section Staining). Gel Shift. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: Lot specific

Purification: Purified

Buffer: 0.1 M Tris-Glycine, pH 7.4, 150 mM Sodium Chloride, 30% Glycerol and0.08% Sodium Azide as preservative.

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only