KRT14 antibody

Catalog No. ABIN264414

Product Details anti-KRT14 Antibody

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts specifically with Human Cytokeratin-14 (50 kDa) by Immunoblotting. Does not react with other Cytokeratins or other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Cytokeratin-14, CK14, Keratin 14, K14, KRT14, Keratin Type I Cytoskeletal 14

Purification: Protein A/G Chromatography

Immunogen: Synthetic peptide conjugated to KLH corresponding to thecarboxy terminal sequence of human cytokeratin 14(KVVSTHEQVLRTKN)Genename: KRT14

Clone: DE-SPK14

Isotype: IgG2b

Application Details

Application Notes: Western Blot: Detects human cytokeratin 14 at 50 kDa. Immunohistochemistry (Frozen Sections): It provides a positive reaction on basal cells ofnon-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis andgingiva, myoepithelial cells and squamous cell carcinomas. Not suitable for use in Paraffin embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14 (KRT14 Products)

Synonyms: CK14 antibody, EBS3 antibody, EBS4 antibody, K14 antibody, NFJ antibody, AI626930 antibody, Krt-1.14 antibody, Krt1-14 antibody, Ka14 antibody, ouro2 antibody, keratin 14 antibody, keratin 14, type I L homeolog antibody, KRT14 antibody, Krt14 antibody, krt14.L antibody

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14

Gene ID: 3861

UniProt: P02533