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Antibodies
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anti-Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Pol...
Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1) antibody
| Antigen |
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| Clonality |
Monoclonal (R4-5) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN264424 |
| Quantity |
100 µg (Lot specific) |
| Price |
440.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: GNAI1, Guanine nucleotide-binding protein G(i) alpha-1 subunit
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Alternative name
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G protein alpha inhibitor 1
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Gene ID
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2770
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Swiss-Prot
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P63096
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Immunogen
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Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized withpartially purified rat brain Gai1 protein and mouse myeloma Ag8 (NS1) cells.
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Cross-Reactivity
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Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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R4-5
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Description
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GTP-binding proteins (G-proteins) play a critical role in signal transduction by couplingreceptors to effectors. The intracellular consequences of G-protein activation includesecond messenger generation (such as cyclic AMP, calcium and diacylglycerol), proteinphosphorylation, ion channel activation, gene induction, cell growth, and differentiation.
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Specificity
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Recognizes a single band of 39-42kDa, identified as the G i1 protein. This antibody is highly specific to Gi alpha1 and shows no cross-reaction with transducin, Galphao, Gi alpha2, Gi alpha3, or Gis. ADP-ribosylation of alphai1 protein does not alter the reactivity of this antibody. Species: Human, Rat, Mouse, Cow and Guinea pig. Others not tested.
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Application Notes
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Western Blot (1-5 µg/ml). Positive Control: IMR-5 cells or brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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Lot specific
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Purification
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Purified
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Buffer
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PBS containing 0.08% Sodium Azide as preservative.
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Storage
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Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 1 (GNAI1)", type "Antibodies"
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