X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody

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Antigen
Synonyms RCC, Xrcc-1, xrcc1, MGC80909, XRCC1, rcc
Reactivity
Human
(76), (26), (26), (1)
Host
Mouse
(47), (30)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(66), (26), (26), (20), (17), (15), (10), (8), (6), (3), (2)
Pubmed 8 references available
Catalog no. ABIN264445
Quantity 0.1 mg
Price
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Immunogen Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant human XRCC1 protein and mouse myeloma Sp2/0 cells.
Clone 2F11
Isotype IgG2a
Characteristics Synonyms: XRCC-1, DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
Purification Purified
Alternative Name XRCC1
Background The C-terminal 96 amino acids of XRCC1 are necessary and sufficient for the specificinteraction with DNA ligase III of 103kDa. The region of interaction with DNA polymerasebeta is located within residues 84-183 in the N-terminal half of the XRCC1 protein. Reportedly, Poly (ADP-ribose) polymerase also interacts with XRCC1. In testis XRCC1 is expressed at high levels. The XRCC1 gene plays a role for the sensitivity of mammalian celllines toward ionizing irradiation. Cells with a mutation of this gene present with decreasedsingle strand break repair and reduced recombination repair, they show increased doublestrand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 mightserve as a scaffold protein during base excision-repair.
UniProt P18887
Research Area Cancer, DNA/RNA
Application Notes Western Blot (4 ug/ml). Immunohistochemistry on Frozen and Formalin/paraffin embedded tissues (2-4 ug/ml). Positive Control: HT29, LS174T, HeLa cells, normal testis. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.08% Sodium Azide as preservative
Preservative Sodium azide
Storage -20 °C
Storage Comment Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody
Background publications Caldecott, Tucker, Stanker et al.: "Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells." in: Nucleic acids research, Vol. 23, Issue 23, pp. 4836-43, 1996 (PubMed).

Andersson, Sadeghi, Siciliano et al.: "Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs." in: Cancer chemotherapy and pharmacology, Vol. 38, Issue 5, pp. 406-16, 1996 (PubMed).

Walter, Trolian, McFarland et al.: "Xrcc-1 expression during male meiosis in the mouse." in: Biology of reproduction, Vol. 55, Issue 3, pp. 630-5, 1997 (PubMed).

Caldecott, Aoufouchi, Johnson et al.: "XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro." in: Nucleic acids research, Vol. 24, Issue 22, pp. 4387-94, 1997 (PubMed).

Kubota, Nash, Klungland et al.: "Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein." in: The EMBO journal, Vol. 15, Issue 23, pp. 6662-70, 1997 (PubMed).

Labudova, Hardmeier, Rink et al.: "The transcription of the XRCC1 gene in the heart of radiation-resistant and radiation-sensitive mice after ionizing irradiation." in: Pediatric research, Vol. 41, Issue 3, pp. 435-9, 1997 (PubMed).

Nash, Caldecott, Barnes et al.: "XRCC1 protein interacts with one of two distinct forms of DNA ligase III." in: Biochemistry, Vol. 36, Issue 17, pp. 5207-11, 1997 (PubMed).

Cappelli, Taylor, Cevasco et al.: "Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair." in: The Journal of biological chemistry, Vol. 272, Issue 38, pp. 23970-5, 1997 (PubMed).

Hosts (47), (30)
Reactivities (76), (26), (26), (1)
Applications (66), (26), (26), (20), (17), (15), (10), (8), (6), (3), (2)
Conjugates (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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