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X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody

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Synonyms xrcc1, MGC80909, XRCC1, rcc, RCC, Xrcc-1
(121), (27), (24), (5)
(82), (39)
Clonality (Clone)
Monoclonal ()
(5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(100), (37), (35), (35), (27), (24), (21), (21), (11), (2), (2), (1)
Pubmed 8 references available
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Quantity 0.1 mg
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Immunogen Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized withrecombinant human XRCC1 protein and mouse myeloma Sp2/0 cells.
Clone 144
Isotype IgG2a
Specificity Recognizes a protein of 85 kDa, identified as the X-Ray Repair Cross Complementing (XRCC1).
Characteristics Synonyms: XRCC-1, DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
Purification Purified
Alternative Name XRCC1 (XRCC1 Antibody Abstract)
Background The C-terminal 96 amino acids of XRCC1 are necessary and sufficient for the specific interaction with DNA ligase III of 103 kDa. The region of interaction with DNA polymerase beta is located within residues 84-183 in the N-terminal half of the XRCC1 protein. Reportedly, Poly (ADP-ribose) polymerase also interacts with XRCC1. In testis XRCC1 is expressed at high levels. The XRCC1 gene plays a role for the sensitivity of mammalian cell lines toward ionizing irradiation. Cells with a mutation of this gene present with decreased single strand break repair and reduced recombination repair, they show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.Synonyms: DNA repair protein XRCC1, X-ray repair cross-complementing protein 1
Gene ID 7515
UniProt P18887
Research Area Cancer, DNA/RNA
Pathways DNA Damage Repair
Application Notes Western Blot (4 μg/mL). Immunohistochemistry on Frozen and Formalin/paraffin embedded tissues (2-4 μg/mL). Positive Control: HT29, LS174T, HeLa cells, normal testis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.08 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody (ABIN264445) anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1) antibody
Background publications Cappelli, Taylor, Cevasco et al.: "Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair." in: The Journal of biological chemistry, Vol. 272, Issue 38, pp. 23970-5, 1997 (PubMed).

Nash, Caldecott, Barnes et al.: "XRCC1 protein interacts with one of two distinct forms of DNA ligase III." in: Biochemistry, Vol. 36, Issue 17, pp. 5207-11, 1997 (PubMed).

Labudova, Hardmeier, Rink et al.: "The transcription of the XRCC1 gene in the heart of radiation-resistant and radiation-sensitive mice after ionizing irradiation." in: Pediatric research, Vol. 41, Issue 3, pp. 435-9, 1997 (PubMed).

Kubota, Nash, Klungland et al.: "Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein." in: The EMBO journal, Vol. 15, Issue 23, pp. 6662-70, 1997 (PubMed).

Caldecott, Aoufouchi, Johnson et al.: "XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro." in: Nucleic acids research, Vol. 24, Issue 22, pp. 4387-94, 1997 (PubMed).

Walter, Trolian, McFarland et al.: "Xrcc-1 expression during male meiosis in the mouse." in: Biology of reproduction, Vol. 55, Issue 3, pp. 630-5, 1997 (PubMed).

Andersson, Sadeghi, Siciliano et al.: "Nucleotide excision repair genes as determinants of cellular sensitivity to cyclophosphamide analogs." in: Cancer chemotherapy and pharmacology, Vol. 38, Issue 5, pp. 406-16, 1996 (PubMed).

Caldecott, Tucker, Stanker et al.: "Characterization of the XRCC1-DNA ligase III complex in vitro and its absence from mutant hamster cells." in: Nucleic acids research, Vol. 23, Issue 23, pp. 4836-43, 1996 (PubMed).

Catalog No. ABIN264445
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