GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN264457, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
628
5
5
2
2
2
1
1
Host
Mouse
323
168
79
40
16
8
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
32
30
26
14
12
11
11
8
8
8
7
7
6
5
5
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
512
329
186
141
137
115
92
71
58
26
15
13
10
8
8
6
6
5
4
4
3
3
3
3
2
2
2
1
1
1
1
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Immunogen Hybridoma produced by the fusion of splenocytes of mice immunized with full-length greenfluorescent protein and mouse myeloma cells.
Clone F56-BA1-2-3
Isotype IgG
Characteristics Synonyms: Green fluorescent protein, GFP-Tag
Purification Purified
Alternative Name GFP
Target Type Tag
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
Research Area Tags/Labels
Application Notes Western Blot (5-10 μg/mL). Positive Control: Purified Green Fluorescent Protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing containing 0.08 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Expiry Date 12 months
Background publications Inouye, Tsuji: "Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein." in: FEBS letters, Vol. 341, Issue 2-3, pp. 277-80, 1994 (PubMed).

Cody, Prasher, Westler, Prendergast, Ward: "Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein." in: Biochemistry, Vol. 32, Issue 5, pp. 1212-8, 1993 (PubMed).

Prasher, Eckenrode, Ward, Prendergast, Cormier: "Primary structure of the Aequorea victoria green-fluorescent protein." in: Gene, Vol. 111, Issue 2, pp. 229-33, 1992 (PubMed).