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Thymidylate Synthetase (TYMS) antibody

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(191), (26), (26), (1), (1), (1)
(109), (70), (14)
Clonality (Clone)
Monoclonal ()
(3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(135), (70), (66), (59), (55), (52), (35), (12), (11), (5), (5), (3), (1), (1)
Pubmed 7 references available
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Quantity 0.1 mg
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Immunogen Hybridoma produced by the fusion of splenocytes from mice immunized with recombinanthuman thymidylate synthase enzyme and mouse myeloma cells.
Clone TS106
Isotype IgG1
Characteristics Synonyms: TYMS, TSase, OK/SW-cl.29
Purification Protein A/G Chromatography.
Alternative Name Thymidylate synthase (TS) (TYMS Antibody Abstract)
Background Thymidylate Synthase (TS) (1) (EC 2. 1. 1. 45) converts deoxyuridine monophosphate (dUMP)to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS isa critical target for the fluoropyrimidines, an important group of antineoplastic drugs thatare widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine areconverted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalentcomplex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expressionof TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal (4,5),gastric (5), head and neck (6) and breast (7) carcinomas.
UniProt P04818
Research Area Enzymes
Application Notes ELISA: This antibody can be used as a Coating. (Ref.1,3)Western Blot (1-2 µg/ml). Immunoprecipitation (2 µg/mg of protein lysate). Immunofluorescense. Flow Cytometry. Immunohistochemistry on Formalin/Paraffin Section (10-20 µg/ml). Staining offormalin-fixed tissues requires boiling in 10 mM citrate buffer, pH 6.0 for 10-20 min andcooling at RT for 20 min. Positive Control: 5-Fluorouracril resistant cell cancer lines or colon carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing containing 0.08% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Thymidylate Synthetase (TYMS) antibody (ABIN264458) anti-Thymidylate Synthetase (TYMS) antibody
Background publications Pestalozzi, Peterson, Gelber et al.: "Prognostic importance of thymidylate synthase expression in early breast cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 15, Issue 5, pp. 1923-31, 1997 (PubMed).

Johnston, Mick, Recant et al.: "Thymidylate synthase expression and response to neoadjuvant chemotherapy in patients with advanced head and neck cancer." in: Journal of the National Cancer Institute, Vol. 89, Issue 4, pp. 308-13, 1997 (PubMed).

Johnston, Drake, Steinberg et al.: "Quantitation of thymidylate synthase in human tumors using an ultrasensitive enzyme-linked immunoassay." in: Biochemical pharmacology, Vol. 45, Issue 12, pp. 2483-6, 1993 (PubMed).

Johnston, Fisher, Rockette et al.: "The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 12, Issue 12, pp. 2640-7, 1995 (PubMed).

Johnston, Lenz, Leichman et al.: "Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors." in: Cancer research, Vol. 55, Issue 7, pp. 1407-12, 1995 (PubMed).

Johnston, Liang, Henry et al.: "Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissue." in: Cancer research, Vol. 51, Issue 24, pp. 6668-76, 1992 (PubMed).

Johnston, Drake, Trepel et al.: "Immunological quantitation of thymidylate synthase using the monoclonal antibody TS 106 in 5-fluorouracil-sensitive and -resistant human cancer cell lines." in: Cancer research, Vol. 52, Issue 16, pp. 4306-12, 1992 (PubMed).

Catalog No. ABIN264458

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