Internexin Neuronal Intermediate Filament Protein, alpha (INA) antibody

Details for Product No. ABIN264466
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Antigen
Reactivity
Mammalian
(77), (49), (37), (15), (13), (13), (12), (2), (1)
Host
Mouse
(68), (34), (13), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(108), (49), (39), (34), (32), (24), (10), (10), (4), (3), (2), (2)
Pubmed 5 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN264466
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Immunogen Hybridoma produced by the fusion of splenocytes from miceimmunized with recombinant rat alpha-internexin and mousemyeloma cells.
Clone 2E3
Isotype IgG1
Characteristics Synonyms: Alpha-Inx, INA, NEF5, NF66, 66 kDa neurofilament protein, Neurofilament-66
Purification Purified
Alternative Name Alpha-Internexin
Background Alpha-internexin is a Class IV intermediathee filament originally discovered as it copurifieswith other neurofilament subunits (1). On SDSPAGE gels it runs with an apparent molecular weight of 64 to 66 kDa, with somespecies variability, although the real molecular weight is about 55kDa, as with the otherneurofilament subunits the presence of highly negatively charged sequences results inreduction of SDS-PAGE mobility. a-internexin is related to but distinct from the betterknown neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar proteinsequence motifs and a similar intron organization. It is expressed only in neurons and inlarge amounts early in neuronal development, but is downregulated in many neurons asdevelopment procedes. Many classes of mature neurons contain a-internexin in additionto NFL, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilamentsubunit expressed. Antibodies to a-internexin are therefore unique probes to study andclassify neuronal types and follow their processes in sections and in tissue culture. Inaddition the very early developmental expression of a-internexin means its presence is anearly and convenient diagnostic feature of neuronal progenitors cells and other cellcommitted to the neuronal lineage. In addition recent studies show a marked upregulationof a-internexin during neuronal regeneration (2).
UniProt Q16352
Application Notes Western Blot: 1: 10,000. Immunohistochemistry. Immunocytochemistry on frozen and paraffin sections: 1: 5000. Positive Control: Human alpha-internexin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The antibody can be shipped at 2-8°C. Store (in aliquots) at -20°C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Pachter, Liem: "alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues." in: The Journal of cell biology, Vol. 101, Issue 4, pp. 1316-22, 1985 (PubMed).

Evans, Sumners, Moore et al.: "Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem." in: Journal of neurophysiology, Vol. 87, Issue 2, pp. 1076-85, 2002 (PubMed).

McGraw, Mickle, Shaw et al.: "Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 12, pp. 4955-63, 2002 (PubMed).

Rajasalu, Teesalu, Janmey et al.: "Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals." in: Immunology letters, Vol. 94, Issue 1-2, pp. 153-60, 2004 (PubMed).

Cairns, Grossman, Arnold et al.: "Clinical and neuropathologic variation in neuronal intermediate filament inclusion disease." in: Neurology, Vol. 63, Issue 8, pp. 1376-84, 2004 (PubMed).

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