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CXCR4 antibody (Chemokine (C-X-C Motif) Receptor 4)

Details for Product anti-CXCR4 Antibody No. ABIN264477, Supplier: Log in to see
Antigen
  • CXC-R4
  • CXCR-4
  • cd184
  • d2s201e
  • fb22
  • hm89
  • hsy3rr
  • lap3
  • lcr1
  • lestr
  • npy3r
  • npyr
  • npyrl
  • npyy3r
  • whim
  • xcxcr4
  • cxcr4
  • CD184
  • Cmkar4
  • LESTR
  • PB-CKR
  • PBSF/SDF-1
  • Sdf1r
  • D2S201E
  • FB22
  • HM89
  • HSY3RR
  • LAP3
  • LCR1
  • NPY3R
  • NPYR
  • NPYRL
  • NPYY3R
  • WHIM
  • CXC-R4-B
  • CXCR-4-B
  • CXCR4
  • cb403
  • zgc:109863
Reactivity
Chimpanzee, Human, Monkey
296
136
72
12
5
4
4
4
3
2
2
2
1
1
1
1
Host
Mouse
214
74
34
28
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CXCR4 antibody is un-conjugated
15
9
7
6
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
214
114
96
69
64
38
31
20
13
12
9
8
7
6
3
2
2
2
2
2
1
1
Supplier
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Immunogen SIV-infected SupT1 cells injected into Balb/c mice.
Clone 12G5
Isotype IgG1
Specificity This antibody recognizes CXC Chemokine Receptor 4 (CXCR4).
Characteristics Synonyms: CXC-R4, CXCR-4, Fusin, LCR1, FB22, NPYRL, HM89, SDF1 receptor, LESTR, C-X-C chemokinereceptor type 4, Stromal cell-derived factor 1 receptor, Leukocyte-derived seventransmembrane domain receptor
Purification Affinity Chromatography.
Alternative Name CD184 / CXCR4 (CXCR4 Antibody Abstract)
Background A receptor for the C-X-C chemokine CXCL12/SDF-1, CXCR4 transduces a signal by increasing the intracellular calcium ions level. It is involved in haematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodelling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. Clone 12G5 has been shown to block infection by certain isolates of HIV-1 and HIV-2 and is specific for CXCR4 and does not react with IL8R-A, CCR1, CCR2b, CCR3, CCR4, and CCR5. CXCR4 is upregulated in response to activation of PBMCs for 3-5 days with PHA.Synonyms: C-X-C chemokine receptor type 4, CXC-R4, CXCR-4, FB22, Fusin, HM89, LCR1, LESTR, Leukocyte-derived seven transmembrane domain receptor, NPYRL, SDF1 receptor, Stromal cell-derived factor 1 receptor
Gene ID 7852
UniProt P61073
Research Area CD Antigens, Surface Receptors of Immune Cells, Chemokines, Receptors
Pathways
Application Notes Flow CytometryImmunofluorescence. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections ( 10 μg/mL). Immunohistochemical analyses of the CXCR4 receptor in Human cell lines and tissues. Fixation with methanol and/or acetone was shown to destroy the epitope of the 12G5 mAb.12G5 can be used to block SDF-1-induced chemotaxis and increases of intracellularcalcium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS, pH 7.3
Storage -20 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-CXCR4 antibody (Chemokine (C-X-C Motif) Receptor 4) (ABIN264477) anti-Chemokine (C-X-C Motif) Receptor 4 (CXCR4) antibody
Background publications Yoshino, Ami, Terao et al.: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

Bleul, Wu, Hoxie et al.: "The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 5, pp. 1925-30, 1997 (PubMed).

Endres, Clapham, Marsh et al.: "CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." in: Cell, Vol. 87, Issue 4, pp. 745-56, 1997 (PubMed).