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Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibody

Details for Product No. ABIN264523, Supplier: Login to see
Antigen
  • AI323664
  • BB161688
  • SLP-76
  • SLP76
  • twm
  • Slp76
Alternatives
anti-Human Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
133
56
42
10
4
3
1
1
1
Host
Mouse
79
38
21
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
109
39
29
23
15
11
8
7
5
4
2
2
1
Supplier
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Immunogen Recombinant human SLP-76 fusion protein
Clone AS55
Isotype IgG2a
Specificity Recognizes human SH2 (Src homology 2) domain-Leukocyte Protein of 76 kDa.
Characteristics Synonyms: LCP2, Lymphocyte cytosolic protein 2, SLP-76 tyrosine phosphoprotein, SLP76, SH2domain-containing leukocyte protein of 76 kDa
Purification Protein G Chromatography
Purity > 95 % by SDS-PAGE
Alternative Name LCP2 (LCP2 Antibody Abstract)
Background SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.Synonyms: LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76
Gene ID 3937
UniProt Q13094
Research Area Signaling
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Western Blot: 1g/mL to visualize 1 x 10e5 Jurkat cells per lane. Immunoprecipitation: 1 to 5 g per 1 x 10^6 Jurkat cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline with 0.1 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibody (ABIN264523) anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) antibody
Background publications Bubeck Wardenburg, Fu, Jackman et al.: "Phosphorylation of SLP-76 by the ZAP-70 protein-tyrosine kinase is required for T-cell receptor function." in: The Journal of biological chemistry, Vol. 271, Issue 33, pp. 19641-4, 1996 (PubMed).

Jackman, Motto, Sun et al.: "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7029-32, 1995 (PubMed).