P21-Activated Kinase 4 (PAK4) (Middle Region) antibody

Details for Product No. ABIN264682
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Antigen
Synonyms 5730488L07Rik, AW555722, mKIAA1142
Epitope
Middle Region
(21), (12), (7), (6), (6), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(112), (59), (50), (38), (37), (37), (13), (2)
Host
Rabbit
(99), (16), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB)
(85), (46), (31), (30), (18), (8), (8), (3), (3), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN264682
544.50 $
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Immunogen Synthetic peptide corresponding to 13 amino acids near the center of human PAK4
Specificity This antibody Reacts to the middle region of PAK4. The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. PAK4 proteins are not activated by Cdc42/Rac binding. PAK4 is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development, and have also implicated PAK4 in anchorage-independent growth of tumor cells.
Purification Immunoaffinity chromatography
Alternative Name PAK4
Background P21-activated kinases (PAKs) belong to the family of serine/threonine kinases involved in the control of various cellular processes, including the cell cycle, dynamics of the cytoskeleton, apoptosis, oncogenic transformation, and transcription. All PAK family members are characterized by the presence of p21-binding domain. p21-activated kinases are regulated by the small GTP-binding proteins Rac and Cdc42, and lipids, which stimulate autophosphorylation and phosphorylation of exogenous substrates. Serine (Ser-474) is the likely autophosphorylation site in the kinase domain of PAK4 in vivo. Phosphospecific antibodies directed against serine 474 detect activated PAK4 on the Golgi membrane when PAK4 is co-expressed with activated Cdc42. Current data strongly implicates PAK-4 in oncogenesis. PAK4 is frequently overexpressed in human tumor cell lines of various tissue origins.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
Gene ID 10298
NCBI Accession NP_001014831
UniProt O96013
Research Area Cancer, Cell Cycle, Cytoskeleton, Microfilaments, Apoptosis/Necrosis, Kinases/Phosphatases
Application Notes Western blot: 1 - 2g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer BPS containing 0.02 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Abo, Qu, Cammarano et al.: "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia." in: The EMBO journal, Vol. 17, Issue 22, pp. 6527-40, 1999 (PubMed).

Qu, Li, Novitch et al.: "PAK4 kinase is essential for embryonic viability and for proper neuronal development." in: Molecular and cellular biology, Vol. 23, Issue 20, pp. 7122-33, 2003 (PubMed).

Li, Minden: "PAK4 functions in tumor necrosis factor (TNF) alpha-induced survival pathways by facilitating TRADD binding to the TNF receptor." in: The Journal of biological chemistry, Vol. 280, Issue 50, pp. 41192-200, 2005 (PubMed).

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