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Details for Product No. ABIN264844

DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1) antibody

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Antigen
Synonyms
HSPF1, Hsp40, 0610007I11Rik, Hdj1, RSPH16B, Sis1, DNAJB1P, HSP40, psiHSP40, CG10578, DNAJ-1, DNAJ1, DROJ1, Dmel\\CG10578, DnaJ1 64EF, DroJ1, EU3500, HSP40/HDJ1, Hsp-40, anon-WO0140519.166, anon-WO0172 ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN264844
Quantity 0.1 mL
Price
368.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant purified Human HSP40 (1)
Specificity Detects 40kDa protein corresponding to the molecular mass of hsp40 on SDS PAGE immunoblots, also detects recombinant hsp40.
Purification Serum
Alternative Name DNAJB1 / HSP40
Background DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).
Alternate names: DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HDJ1, HSP-40, HSPF1, Heat shock 40 kDa protein 1
Gene ID 3337
NCBI Accession NP_006136.1
UniProt P25685
Application Notes Western blot (1: 1,500-2,000). Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Hattori, Liu, Tohnai et al.: "Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells." in: Cell structure and function, Vol. 17, Issue 1, pp. 77-86, 1992 (PubMed).

Ohtsuka, Masuda, Nakai et al.: "A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells." in: Biochemical and biophysical research communications, Vol. 166, Issue 2, pp. 642-7, 1990 (PubMed).

Ohki, Tamura, Nishimura et al.: "Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1778-81, 1986 (PubMed).

Bardwell, Tilly, Craig et al.: "The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1782-5, 1986 (PubMed).

Ohtsuka: "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of bacterial DnaJ." in: Biochemical and biophysical research communications, Vol. 197, Issue 1, pp. 235-40, 1994 (PubMed).

Melville, Hansen, Freeman et al.: "The molecular chaperone hsp40 regulates the activity of P58IPK, the cellular inhibitor of PKR." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 97-102, 1997 (PubMed).

Zhong, Ding, Adams et al.: "Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones." in: Genes & development, Vol. 23, Issue 4, pp. 482-95, 2009 (PubMed).

Alternatives for antigen "DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1)", type "Antibodies"
Hosts (58), (31)
Reactivities (86), (30), (29), (4), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (87), (40), (27), (26), (23), (16), (10), (9), (3), (2), (2), (1)
Conjugates (3), (3), (1), (1), (1)
Epitopes (11), (11), (4), (4), (1)
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