SOD1 antibody

Catalog No. ABIN264854

Product Details anti-SOD1 Antibody

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: Detects a 23 kDa (Human) and 19 kDa (other species) proteins corresponding to the Molecular Mass of Cu/Zn Superoxide Dismutase (SOD) on SDS-PAGE Immunoblots.

Purification: Antigen Affinity Chromatography

Immunogen: Rat Cu/Zn SOD

Isotype: IgG

Application Details

Application Notes: Western blot: 0.5 μg/mL was sufficient for detection of Cu/Zn SOD in 20 μg of rat braintissue extract by Colorimetric Immunoblot analysis using Goat anti-RabbitIgG-AP as the secondary antibody. (Ref.6)Immunoprecipitation. EIA (Ref.6). Immunohistochemistry on Frozen Sections (Ref.8).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.0, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SOD1 antibody (ABIN264854)

Zuber, Rappaport, Luo, Wang, Chen, Vaseva, Shi, Weissmueller, Fellmann, Fellman, Taylor, Weissenboeck, Graeber, Kogan, Vakoc, Lowe: "An integrated approach to dissecting oncogene addiction implicates a Myb-coordinated self-renewal program as essential for leukemia maintenance." in: Genes & development, Vol. 25, Issue 15, pp. 1628-40, (2011) (PubMed).

Wang, Kumagai-Braesch, Herrington, Larsson, Permert: "Increased lipid metabolism and cell turnover of MiaPaCa2 cells induced by high-fat diet in an orthotopic system." in: Metabolism: clinical and experimental, Vol. 58, Issue 8, pp. 1131-6, (2009) (PubMed).

Huang, Lee, Kuo, Yang, Chien: "Attenuation of long-term Rhodiola rosea supplementation on exhaustive swimming-evoked oxidative stress in the rat." in: The Chinese journal of physiology, Vol. 52, Issue 5, pp. 316-24, (2009) (PubMed).

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: Superoxide Dismutase 1 / SOD1 (SOD1 Products)

Synonyms: LOC692639 antibody, ALS antibody, ALS1 antibody, IPOA antibody, SOD antibody, hSod1 antibody, homodimer antibody, CG11793 antibody, Cu antibody, Cu-Zn SOD antibody, Cu/Zn SOD antibody, Cu/Zn sod antibody, Cu/Zn superoxide dismutase antibody, Cu/ZnSOD antibody, CuSOD antibody, CuZn SOD antibody, CuZn-SOD antibody, CuZn-SOD1 antibody, CuZnSOD antibody, Cu[2+]/Zn[2+]SOD antibody, Dmel\\CG11793 antibody, G antibody, Mn SOD antibody, SOD-1 antibody, SOD1 antibody, Sod-1 antibody, Sod1 antibody, To antibody, To-1 antibody, Zn SOD antibody, Zn Sod antibody, Zn-SOD antibody, ZnSod antibody, cSOD antibody, cSod antibody, dSOD1 antibody, l(3)108 antibody, l(3)68Af' antibody, l(3)G antibody, sod antibody, sod1 antibody, CU/ZN-SOD antibody, SODC antibody, DKFZP469M1833 antibody, B430204E11Rik antibody, Cu/Zn-SOD antibody, Ipo-1 antibody, Ipo1 antibody, SOD1L1 antibody, XSODB antibody, als antibody, als1 antibody, ipoa antibody, sod1-a antibody, ZSOD antibody, cuzn antibody, Cu/Zn superoxide dismutase antibody, superoxide dismutase 1 antibody, Superoxide dismutase 1 antibody, superoxide dismutase 1, soluble antibody, superoxide dismutase 1 S homeolog antibody, Superoxide dismutase [Cu-Zn] antibody, superoxide dismutase 1 L homeolog antibody, superoxide dismutase [Cu-Zn]-like antibody, superoxide dismutase [Cu-Zn] antibody, superoxide dismutase Sod1 antibody, SOD antibody, SOD1 antibody, Sod1 antibody, sod1 antibody, sod1.S antibody, sod-1 antibody, sod1.L antibody, LOC101451855 antibody, LOC101115136 antibody

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (Ref.3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (Ref.2,5). In general, SODs play a major role in antioxidant defense mechanisms. (Ref.4) There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (Ref.3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (Ref.7). SOD3 can also be distinguished by its heparinbinding capacity (Ref.1).Synonyms: ALS1, CuZn-SOD, CuZnSOD, IPOA, SOD-1, Superoxide dismutase [Cu-Zn]

Gene ID: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis