anti-Calnexin antibody (CANX)

Details for Product anti-CANX Antibody No. ABIN264868
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Synonyms zgc:63524, wu:fe06b12, clgn, clnx, cnx, CANX, CNX, IP90, P90, canx, 1110069N15Rik, AI988026, Cnx, D11Ertd153e, PP90, CG11958, Dmel\CG11958
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Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(227), (123), (117), (93), (73), (69), (68), (68), (67), (66), (65), (65), (62), (39), (39), (6), (5), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1)
(201), (29), (22)
This Calnexin antibody is un-conjugated
(7), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(234), (140), (116), (100), (89), (75), (48), (36), (10), (9), (8), (5), (2), (1), (1), (1)
Pubmed 10 references available
Quantity 0.2 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen A 19 residue synthetic peptide based on Canine Calnexin and the peptide coupled to KLH
Isotype IgG
Specificity This antibody detects Calnexin at ~90 kDa.
Purification Serum
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene ID 821
NCBI Accession NP_001019820
UniProt P27824
Pathways MAPK Signaling
Application Notes Western blot: A 1/5000-10000 dilution is sufficient for detection of Calnexin in 20 μg ofHela cell lysate by ECL Immunoblot analysis. Immunoprecipitation: 1/100 (Ref.9). Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
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Background publications Liu, Milner, Boppart et al.: "β1D chain increases α7β1 integrin and laminin and protects against sarcolemmal damage in mdx mice." in: Human molecular genetics, Vol. 21, Issue 7, pp. 1592-603, 2012 (PubMed).

Janiszewski, Lopes, Carmo et al.: "Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 280, Issue 49, pp. 40813-9, 2005 (PubMed).

Raggo, Rapin, Stirling et al.: "Luman, the cellular counterpart of herpes simplex virus VP16, is processed by regulated intramembrane proteolysis." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5639-49, 2002 (PubMed).

Schrag, Bergeron, Li et al.: "The Structure of calnexin, an ER chaperone involved in quality control of protein folding." in: Molecular cell, Vol. 8, Issue 3, pp. 633-44, 2001 (PubMed).

Elagöz, Callejo, Armstrong et al.: "Although calnexin is essential in S. pombe, its highly conserved central domain is dispensable for viability." in: Journal of cell science, Vol. 112 ( Pt 23), pp. 4449-60, 2000 (PubMed).

Rubio, Wenthold: "Calnexin and the immunoglobulin binding protein (BiP) coimmunoprecipitate with AMPA receptors." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 942-8, 1999 (PubMed).

Otteken, Moss: "Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 97-103, 1996 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Tjoelker, Seyfried, Eddy et al.: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, 1994 (PubMed).

Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).

Catalog No. ABIN264868
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