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Calnexin (CANX) antibody

Details for Product No. ABIN264869
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Antigen
Synonyms CNX, IP90, P90, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, PP90, CG11958, Dmel\\CG11958, cnx, canx, clgn, clnx, zgc:63524, wu:fe06b12, CANX, fj49d10, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042
Reactivity
Cow (Bovine), Dog (Canine), Chicken, Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(122), (46), (44), (25), (25), (23), (9), (8), (8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Host
Rabbit
(108), (23), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(132), (50), (43), (42), (40), (38), (33), (26), (10), (9), (1), (1)
Pubmed 10 references available
Quantity 50 µL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN264869
253.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A 19 residue synthetic peptide based on Canine Calnexin and the peptide coupled to KLH
Isotype IgG
Specificity This antibody detects Calnexin at ~90kDa.
Purification Serum
Alternative Name Calnexin
Background Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium- binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).
Alternate names: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Gene ID 821
NCBI Accession NP_001019820.1
UniProt P27824
Application Notes Western blot: A 1/5000-10000 dilution is sufficient for detection of Calnexin in 20 µg ofHela cell lysate by ECL Immunoblot analysis. Immunoprecipitation: 1/100 (Ref.9). Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Calnexin (CANX) antibody anti-Calnexin (CANX) antibody
anti-Calnexin (CANX) antibody (2) anti-Calnexin (CANX) antibody (Image 2)
anti-Calnexin (CANX) antibody (3) anti-Calnexin (CANX) antibody (Image 3)
Background publications Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).

Tjoelker, Seyfried, Eddy et al.: "Human, mouse, and rat calnexin cDNA cloning: identification of potential calcium binding motifs and gene localization to human chromosome 5." in: Biochemistry, Vol. 33, Issue 11, pp. 3229-36, 1994 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Otteken, Moss: "Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin." in: The Journal of biological chemistry, Vol. 271, Issue 1, pp. 97-103, 1996 (PubMed).

Rubio, Wenthold: "Calnexin and the immunoglobulin binding protein (BiP) coimmunoprecipitate with AMPA receptors." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 942-8, 1999 (PubMed).

Elagöz, Callejo, Armstrong et al.: "Although calnexin is essential in S. pombe, its highly conserved central domain is dispensable for viability." in: Journal of cell science, Vol. 112 ( Pt 23), pp. 4449-60, 2000 (PubMed).

Schrag, Bergeron, Li et al.: "The Structure of calnexin, an ER chaperone involved in quality control of protein folding." in: Molecular cell, Vol. 8, Issue 3, pp. 633-44, 2001 (PubMed).

Raggo, Rapin, Stirling et al.: "Luman, the cellular counterpart of herpes simplex virus VP16, is processed by regulated intramembrane proteolysis." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5639-49, 2002 (PubMed).

Janiszewski, Lopes, Carmo et al.: "Regulation of NAD(P)H oxidase by associated protein disulfide isomerase in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 280, Issue 49, pp. 40813-9, 2005 (PubMed).

Liu, Milner, Boppart et al.: "β1D chain increases α7β1 integrin and laminin and protects against sarcolemmal damage in mdx mice." in: Human molecular genetics, Vol. 21, Issue 7, pp. 1592-603, 2012 (PubMed).

Hosts (108), (23), (10)
Reactivities (122), (46), (44), (25), (25), (23), (9), (8), (8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4), (3), (3), (2), (1), (1), (1)
Applications (132), (50), (43), (42), (40), (38), (33), (26), (10), (9), (1), (1)
Conjugates (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (36), (11), (5), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN264869
253.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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