MAGI3 antibody

Catalog No. ABIN264918

Product Details anti-MAGI3 Antibody

Target: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This MAGI3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to M A G I - 3 , P D Z 4 - 5 .

Purification: Purified

Immunogen: Recombinant protein corresponding to PDZ domain 4-5 of MAGI-3.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1 - 10 μg/mL. Detects a 130 kDa band in cells transfected with full-lengthMAGI-3 protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for MAGI3

Target: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Alternative Name: MAGI3 (MAGI3 Products)

Synonyms: Slipr antibody, MAGI-3 antibody, RP4-730K3.1 antibody, dJ730K3.2 antibody, 4732496O19Rik antibody, 6530407C02Rik antibody, AA407180 antibody, AI120132 antibody, mKIAA1634 antibody, membrane associated guanylate kinase, WW and PDZ domain containing 3 antibody, Magi3 antibody, MAGI3 antibody

Background: Membrane-associated guanylate kinase with inverted orientation (MAGI3) is a novel, 130 kDa guanylate kinase which is part of a family of multi-PDZ domain containing guanylate kinases. These guanylate kinases are localized to epithelial cell tight junctions. MAGI3 and PTEN/MMAC cooperate to modulate the kinase activity of AKT/protein kinase B (PKB). MAGI3 positions the PTEN/MMAC phosphatase to specific subcellular locations that are involved with the regulation of cell proliferation and survival. MAGI3, as well as other proteins in this family, are made up of an N-terminal guanylate kinase domain, followed by a WW domain, named for two conserved tryptophan residues and first identified as two repeats in mouse YAP65, which is then followed by 6 PDZ domains.Synonyms: MAGI-3, Membrane-associated guanylate kinase, Membrane-associated guanylate kinase inverted 3, WW and PDZ domain-containing protein 3

Gene ID: 260425

NCBI Accession: NP_001136254

UniProt: Q5TCQ9