anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (AA 12-28)

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Antigen
Synonyms
APAF1, ARK, Apaf-1, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon- ... show more
APAF1, ARK, Apaf-1, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, apaf-1, apaf1, arc, ark, dAPAF-1, dApaf-1, dApaf-1/DARK/HAC-1, dApaf1, dArk, dapaf, dapaf-1, dapaf-1L, dapaf-1S, dark, dark/dapaf-1/hac-1, dark/hac-1/dapaf-1, hac-1, hac1, l(2)SH0173, APAF-1, CED4, 6230400I06Rik, Apaf1l, fog, mKIAA0413, zgc:136543 show less
Alternatives
Epitope
AA 12-28
(51), (48), (17), (12), (12), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(194), (105), (83), (3), (2), (2), (2), (1), (1)
Host
Rabbit
(166), (25), (12), (4)
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
(3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB)
(155), (65), (64), (49), (27), (20), (15), (10), (8), (5), (4)
Pubmed 1 reference available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse
Isotype IgG
Specificity This antibody reacts to APAF1.
Purification Purified
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
NCBI Accession NP_001151
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot: 0.25 to 0.5 μg/mL. Detects a 130 kDa band in whole HeLa cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline solution containing 0.02 % sodium azide as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.
Background publications Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Catalog No. ABIN264991
$390.50
Plus shipping costs $45.00

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