Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 12-28) antibody

Details for Product No. ABIN264991
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Antigen
Synonyms
APAF-1, CED4, 6230400I06Rik, Apaf-1, Apaf1l, fog, mKIAA0413, apaf-1, zgc:136543, APAF1, ARK, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dAp ... show more
APAF-1, CED4, 6230400I06Rik, Apaf-1, Apaf1l, fog, mKIAA0413, apaf-1, zgc:136543, APAF1, ARK, Apaf1, CG6829, D-Apaf-1, DARK, Dapaf-1, Dapaf-1/HAC-1, Dark, Dark/Apaf-I, Dark/Dapaf-1/HAC1, Dark/Hac-1/dApaf-1, Dark/Hac-1/dApaf1, Dmel\\CG6829, Hac-1, Hac-1/Dark, Hac1, T1, anon-53Fa, apaf1, arc, ark, dAPAF-1, dApaf-1, dApaf-1/DARK/HAC-1, dApaf1, dArk, dapaf, dapaf-1, dapaf-1L, dapaf-1S, dark, dark/dapaf-1/hac-1, dark/hac-1/dapaf-1, hac-1, hac1, l(2)SH0173 show less
Epitope
AA 12-28
(46), (35), (8), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(160), (90), (81), (24), (15), (12), (1), (1), (1), (1), (1)
Host
Rabbit
(132), (38), (6), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB)
(124), (58), (52), (46), (20), (20), (14), (11), (7), (4), (3), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN264991
Quantity 0.1 mg
Price
368.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days

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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse
Isotype IgG
Specificity This antibody reacts to APAF1.
Purification Purified
Alternative Name APAF1
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).
Alternate names: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
NCBI Accession NP_001151.1
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Application Notes Western Blot: 0.25 to 0.5 ug/ml. Detects a 130 kDa band in whole HeLa cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffered saline solution containing 0.02% sodium azide as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8°C.
Expiry Date 12 months
Background publications Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Hosts (132), (38), (6), (2)
Reactivities (160), (90), (81), (24), (15), (12), (1), (1), (1), (1), (1)
Applications (124), (58), (52), (46), (20), (20), (14), (11), (7), (4), (3), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (46), (35), (8), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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