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BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L) (AA 77-92) antibody

Details for Product No. ABIN264993, Supplier: Log in to see
Antigen
  • BNIP3a
  • NIX
  • C86132
  • D14Ertd719e
  • Nip3L
  • Nix
Epitope
AA 77-92
19
16
15
9
8
7
4
3
3
2
1
1
1
1
1
1
1
1
Reactivity
Human
92
29
19
1
Host
Rabbit
88
6
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
65
44
40
15
15
12
12
5
4
2
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 77 to 92 of the human Bnip3L protein. Immunogen sequence is identical in the mouse protein.
Isotype IgG
Specificity This antibody recognizes Bnip3L (IN) (nix).
Purification Purified
Alternative Name BNIP3L / BNIP3A (BNIP3L Antibody Abstract)
Background Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (1-3). Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis (2,3). Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (1,2). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.Synonyms: Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, BNIP3H, NIP3-like protein X, NIX
Gene ID 665
NCBI Accession NP_004322
UniProt O60238
Application Notes Western Blot: 0.5 to 1 μg/mL. Detects a 40 kDa band in K562 cell lysate which representsthe monomer of Bnip3L.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as a preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.
Supplier Images
 image for anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L) (AA 77-92) antibody (ABIN264993) anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L) (AA 77-92) antibody
Product cited in: Zhang, Randall, Loyd et al.: "Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation." in: Blood, Vol. 114, Issue 1, pp. 157-64, 2009 (PubMed).

Aerbajinai, Giattina, Lee et al.: "The proapoptotic factor Nix is coexpressed with Bcl-xL during terminal erythroid differentiation." in: Blood, Vol. 102, Issue 2, pp. 712-7, 2003 (PubMed).

Background publications Imazu, Shimizu, Tagami et al.: "Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability." in: Oncogene, Vol. 18, Issue 32, pp. 4523-9, 1999 (PubMed).

Yasuda, Han, Dionne et al.: "BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3." in: Cancer research, Vol. 59, Issue 3, pp. 533-7, 1999 (PubMed).

Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Matsushima, Fujiwara, Takahashi et al.: "Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3." in: Genes, chromosomes & cancer, Vol. 21, Issue 3, pp. 230-5, 1998 (PubMed).