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ADAM17 antibody (ADAM Metallopeptidase Domain 17) (AA 807-823)

Details for Product anti-ADAM17 Antibody No. ABIN264994, Supplier: Log in to see
Antigen
  • adam17
  • adam18
  • ADAM18
  • CD156b
  • cd156b
  • CD156B
  • CSVP
  • csvp
  • NISBD
  • si:dkey-81b15.2
  • tace
  • TACE
  • Tace
Alternatives
anti-Human ADAM17 antibody for Western Blotting
Epitope
AA 807-823, C-Term
82
18
14
14
13
9
7
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
205
64
55
2
2
1
1
1
1
1
Host
Rabbit
170
25
16
1
1
Clonality
Polyclonal
Conjugate
This ADAM17 antibody is un-conjugated
9
8
6
6
5
5
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
173
118
27
26
21
13
12
8
7
5
3
2
1
1
Supplier
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Immunogen Synthetic peptide corresponding to amino acids 807 to 823 of the human TACE protein. Immunogen sequence differs from the mouse and rat sequences by one amino acid.
Isotype IgG
Specificity This antibody Detects TACE (ADAM17/CD156b).
Purification Immunoaffinity Chromatography.
Alternative Name CD156b / ADAM17 (ADAM17 Antibody Abstract)
Background Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a. TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association. (6)Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme
Gene ID 6868
NCBI Accession NP_003174
UniProt P78536
Research Area CD Antigens, Surface Receptors of Immune Cells, Enzymes
Pathways Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot (0.25-1 μg/mL): Detects an approximately 130 kDa band in HeLa and Jurkatwhole cell lysate which may represent mature protein, precursor, and glycosylated TACE.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline solution containing 0.02 % Sodium Azide as a preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Storage 4 °C
Storage Comment The antibody can be shipped at ambient temperature. Store (in aliquots) at 2-8 °C.
Supplier Images
 image for anti-ADAM17 antibody (ADAM Metallopeptidase Domain 17) (AA 807-823) (ABIN264994) anti-ADAM Metallopeptidase Domain 17 (ADAM17) (AA 807-823), (C-Term) antibody
Background publications Mizui, Yamazaki, Sagane, Tanaka: "cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter." in: Gene, Vol. 233, Issue 1-2, pp. 67-74, 1999

Giegel, Chaplin: "Targets in cytokine activation." in: Agents and actions. Supplements, Vol. 49, pp. 1-3, 1998

Peschon, Slack, Reddy, Stocking, Sunnarborg, Lee, Russell, Castner, Johnson, Fitzner, Boyce, Nelson, Kozlosky, Wolfson, Rauch, Cerretti, Paxton, March, Black: "An essential role for ectodomain shedding in mammalian development." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1281-4, 1998

Buxbaum, Liu, Luo, Slack, Stocking, Peschon, Johnson, Castner, Cerretti, Black: "Evidence that tumor necrosis factor alpha converting enzyme is involved in regulated alpha-secretase cleavage of the Alzheimer amyloid protein precursor." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 27765-7, 1998

Black, Rauch, Kozlosky, Peschon, Slack, Wolfson, Castner, Stocking, Reddy, Srinivasan, Nelson, Boiani, Schooley, Gerhart, Davis, Fitzner, Johnson, Paxton, March, Cerretti: "A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells." in: Nature, Vol. 385, Issue 6618, pp. 729-33, 1997

Moss, Jin, Milla, Bickett, Burkhart, Carter, Chen, Clay, Didsbury, Hassler, Hoffman, Kost, Lambert, Leesnitzer, McCauley, McGeehan, Mitchell, Moyer, Pahel, Rocque, Overton, Schoenen, Seaton, Su et al.: "Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. ..." in: Nature, Vol. 385, Issue 6618, pp. 733-6, 1997