BACE1 antibody (AA 485-501)

Catalog No. ABIN264995

Product Details anti-BACE1 Antibody

Target: BACE1 (Beta-secretase 1 (BACE1))

Binding Specificity: AA 485-501

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BACE1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody Detects BACE (CT).

Purification: Purified

Immunogen: Synthetic peptide corresponding to amino acids 485 to 501 of Human BACE.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5-1.0 μg/mL. Detects an approximately 70 kDa band. Positive Control: Human brain tissue lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline solution containing 0.02 % Sodium Azide as a preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for BACE1

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE1 (BACE1 Products)

Background: BACE1 is a protease responsible for the proteolytic processing of the amyloid precursor protein (APP). Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE), of which there are two isoforms, BACE1 and 2. This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide.Synonyms: ASP2, Aspartyl protease 2, BACE, BACE-1, Beta-secretase 1, Beta-site APP cleaving enzyme 1, Beta-site amyloid precursor protein cleaving enzyme 1, KIAA1149, Memapsin-2, Membrane-associated aspartic protease 2

Gene ID: 23621

NCBI Accession: NP_036236

UniProt: P56817