RIT2 antibody

Catalog No. ABIN265015

Product Details anti-RIT2 Antibody

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to RIT2 (Rin).

Purification: Purified

Immunogen: His-tagged recombinant human Rin protein

Isotype: IgG

Application Details

Application Notes: Western Blot: detects recombinant Rin protein expressed in HEK-293 cells, Shows minimalcross-reactivity with Rit protein and other Ras proteins.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for RIT2

Target: RIT2 (Ras-Like Without CAAX 2 (RIT2))

Alternative Name: RIT2 (RIT2 Products)

Synonyms: RIBA antibody, RIN antibody, ROC2 antibody, Rin antibody, Rit1 antibody, Roc2 antibody, RIT2 antibody, Ras like without CAAX 2 antibody, Ras-like without CAAX 2 antibody, RIT2 antibody, Rit2 antibody

Background: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: GTP-binding protein Rit2, RIN, ROC2, Ras-like protein expressed in neurons, Ras-like without CAAX protein 2

Gene ID: 6014

NCBI Accession: NP_002921

UniProt: Q99578

Pathways: Neurotrophin Signaling Pathway