SGPP2
Reactivity: Human
WB, ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Western Blot: 1: 400, Tissue lysate should be used at 10 - 50 g/lane. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Ship on dry ice. Store (in aliquots) at -20 °C only.
Sphingosine 1-phosphate (S1P) is a highly bioactive lipid that has a myriad of biological effects both intracellularly as a second messenger and extracellularly by binding to the S1P(1 - 5)/Gprotein-coupled receptors of the endothelial differentiation gene family. Intracellularly, at least two enzymes, sphingosine kinase (1 and 2) and S1P phosphatase, regulate the activity of S1P by governing the phosphorylation of S1P. SPPase2 is localized to the endoplasmic reticulum. The enzymatic activity and localization of SPPase2 are similar to SPPase1. The tissue expression of SPPase2 was different from that of SPPase1. SPPase2 is an important member of the SPPase family which play a role in attenuating intracellular S1P signaling.Synonyms: SPPase2, Sphingosine-1-phosphatase 2, Sphingosine-1-phosphate phosphatase 2