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MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr292)

Details for Product anti-MAP2K1 Antibody No. ABIN265089, Supplier: Log in to see
Antigen
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
Alternatives
anti-Human MAP2K1 antibody for Western Blotting
Epitope
pThr292
43
37
32
32
31
31
28
27
25
22
18
15
13
13
13
11
9
8
7
7
6
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6
6
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4
4
4
3
3
3
2
2
2
2
2
2
2
2
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2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
Reactivity
Human
578
311
267
56
38
20
14
13
10
6
4
2
2
Host
Rabbit
468
120
2
2
1
Clonality
Polyclonal
Conjugate
This MAP2K1 antibody is un-conjugated
13
13
13
10
9
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7
6
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6
6
6
6
6
1
1
Application
Dot Blot (DB), Western Blotting (WB)
426
200
149
113
87
66
22
21
11
10
9
6
6
2
2
2
2
2
Supplier
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Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr292 of human MEK 1.
Isotype IgG
Specificity This antibody react to MEK1 (pThr292). Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity. :
Purification Affinity Purified
Alternative Name MAP2K1 (MAP2K1 Antibody Abstract)
Background MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Blot: 1: 1000 dilution to provide for 10 miniblots. Dot Blot: 1: 1000 dilution to provide for 10 miniblots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer HEPES ( pH 7.5) solution containing 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Ship on gel ice. Store (in aliquots) at -20 °C to -70 °C.
Supplier Images
 image for anti-MAP2K1 antibody (Mitogen-Activated Protein Kinase Kinase 1) (pThr292) (ABIN265089) anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibody
Background publications Park, Zarrinpar, Lim: "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms." in: Science (New York, N.Y.), Vol. 299, Issue 5609, pp. 1061-4, 2003 (PubMed).

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002 (PubMed).

Mansour, Resing, Candi et al.: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Ahn, Robbins, Haycock et al.: "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin." in: Journal of neurochemistry, Vol. 59, Issue 1, pp. 147-56, 1992 (PubMed).