Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibody

Details for Product No. ABIN265089
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Antigen
Synonyms
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN  ... show more
Mek1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, MEKK1, Prkmk1, mek-2, si:ch211-242m18.2, wu:fj56a12, wu:fj61b01, zgc:56557, ATMEK1, F20B18.180, F20B18_180, MAP kinase/ ERK kinase 1, MITOGEN ACTIVATED PROTEIN KINASE KINASE, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1, NMAPKK, MEK show less
Epitope
pThr292
(32), (27), (25), (25), (24), (22), (21), (20), (19), (12), (12), (11), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(407), (222), (212), (72), (46), (40), (33), (8), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(377), (41), (2), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (10), (10), (6), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (1), (1)
Application
Dot Blot (DB), Western Blotting (WB)
(328), (123), (104), (92), (60), (50), (20), (20), (14), (3), (2), (1)
Pubmed 5 references available
Quantity 1 vial
Quantity Comment 10 blots
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Catalog No. ABIN265089
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Immunogen Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr292 of human MEK 1.
Isotype IgG
Specificity This antibody react to MEK1 (pThr292). Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non- phospho peptide to block the antibody activity.
Purification Affinity Purified
Alternative Name MAP2K1
Background MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386.
Alternate names: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID 5604
NCBI Accession NP_002746
UniProt Q02750
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Application Notes Western Blot: 1: 1000 dilution to provide for 10 miniblots. Dot Blot: 1: 1000 dilution to provide for 10 miniblots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Ship on gel ice. Store (in aliquots) at -20°C to -70°C.
Expiry Date 12 months
Supplier Images
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibody anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibody
Background publications Ahn, Robbins, Haycock et al.: "Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin." in: Journal of neurochemistry, Vol. 59, Issue 1, pp. 147-56, 1992 (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Mansour, Resing, Candi et al.: "Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis." in: Journal of biochemistry, Vol. 116, Issue 2, pp. 304-14, 1995 (PubMed).

Adams, Sweatt: "Molecular psychology: roles for the ERK MAP kinase cascade in memory." in: Annual review of pharmacology and toxicology, Vol. 42, pp. 135-63, 2002 (PubMed).

Park, Zarrinpar, Lim: "Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms." in: Science (New York, N.Y.), Vol. 299, Issue 5609, pp. 1061-4, 2003 (PubMed).

Validation Images
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