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UBE2N / BLU (C-Term) antibody

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(2), (2)
Human, Mouse (Murine), Rat (Rattus)
(5), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1)
(3), (2)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(5), (4), (3), (1)
Pubmed 6 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Peptide corresponding to 15 amino acids near the C-terminus of Human UBC13
Isotype IgG
Specificity This antibody detects UBC13.
Purification Purified
Background Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).Synonyms: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N
Gene ID 7334
NCBI Accession NP_003339
UniProt P61088
Application Notes Western Blot: 0.5-1 μg/mL. Immunohistochemistry. Positive Control: HepG2 Cell Lysate, also Human Small Intestine Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline with 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.
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 image for anti-UBE2N / BLU (C-Term) antibody (ABIN265157) anti-UBE2N / BLU (C-Term) antibody
Background publications Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004 (PubMed).

Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Wang, Deng, Hong et al.: "TAK1 is a ubiquitin-dependent kinase of MKK and IKK." in: Nature, Vol. 412, Issue 6844, pp. 346-51, 2001 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Brusky, Zhu, Xiao: "UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae." in: Current genetics, Vol. 37, Issue 3, pp. 168-74, 2000 (PubMed).

Yamaguchi, Shirakabe, Shibuya et al.: "Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction." in: Science (New York, N.Y.), Vol. 270, Issue 5244, pp. 2008-11, 1996 (PubMed).

Catalog No. ABIN265157
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