UBE2N / BLU (C-Term) antibody

Details for Product No. ABIN265157
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
C-Term
(3), (2)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(6), (6), (6), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(4), (2)
Clonality
Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(6), (5), (5), (3), (1)
Pubmed 6 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN265157
368.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide corresponding to 15 amino acids near the C-terminus of Human UBC13
Isotype IgG
Specificity This antibody detects UBC13.
Purification Purified
Background Ubiquitin-conjugating enzyme 13 (Ubc13) was initially discovered in S. cerevisiae as a DNA-damage inducible protein involved in the error-free DNA postreplication repair pathway (1). It has recently been shown to be an important component of the Toll-like receptor and IL-1R signaling pathway (reviewed in 2). Signals from these pathways are relayed by a number of downstream molecules such as MyD88 and tumor necrosis factor receptor associated factor (TRAF6), ultimately activating various kinases and transcription factors (2,3). Ubc13 is part of a dimeric ubiquitin-conjugating enzyme complex also containing UEV1A (ubiquitin-conjugating enzyme E2 variant 1) that together with TRAF6 activates TAK1, a member of the mitogen-activated protein kinase kinase kinase family (4-6). The Ubc13-UEV1A complex also mediates the Lys-63 ubiquitination of TRAF-6, and this ubiquitination is essential for TAK1 activation (5).Synonyms: Bendless-like ubiquitin-conjugating enzyme, UBC13, Ubiquitin carrier protein N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N
Gene ID 7334
NCBI Accession NP_003339
UniProt P61088
Application Notes Western Blot: 0.5-1 μg/mL. Immunohistochemistry. Positive Control: HepG2 Cell Lysate, also Human Small Intestine Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline with 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Can be shipped at 2-8 °C.
Supplier Images
anti-UBE2N / BLU (C-Term) antibody anti-UBE2N / BLU (C-Term) antibody
Background publications Yamaguchi, Shirakabe, Shibuya et al.: "Identification of a member of the MAPKKK family as a potential mediator of TGF-beta signal transduction." in: Science (New York, N.Y.), Vol. 270, Issue 5244, pp. 2008-11, 1996 (PubMed).

Brusky, Zhu, Xiao: "UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae." in: Current genetics, Vol. 37, Issue 3, pp. 168-74, 2000 (PubMed).

Deng, Wang, Spencer et al.: "Activation of the IkappaB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex and a unique polyubiquitin chain." in: Cell, Vol. 103, Issue 2, pp. 351-61, 2000 (PubMed).

Wang, Deng, Hong et al.: "TAK1 is a ubiquitin-dependent kinase of MKK and IKK." in: Nature, Vol. 412, Issue 6844, pp. 346-51, 2001 (PubMed).

Vogel, Fitzgerald, Fenton: "TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression." in: Molecular interventions, Vol. 3, Issue 8, pp. 466-77, 2004 (PubMed).

Akira, Takeda: "Toll-like receptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 7, pp. 499-511, 2004 (PubMed).

Validation Images
Did you look for something else?
back to top