NOTUM antibody

Catalog No. ABIN265168

Product Details anti-NOTUM Antibody

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTUM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects Notum.

Purification: Purified

Immunogen: Recombinant full-length notum protein from Mouse

Isotype: IgG

Application Details

Application Notes: Western blot: 5-10 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline with 0.08 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the product (in aliquots) at -20 °C. Can be shipped at 2-8 °C.

Target Details for NOTUM

Target: NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Alternative Name: Notum (NOTUM Products)

Synonyms: 5730593N15Rik antibody, RGD1307119 antibody, notum, palmitoleoyl-protein carboxylesterase antibody, notum pectinacetylesterase homolog (Drosophila) antibody, NOTUM, palmitoleoyl-protein carboxylesterase antibody, NOTUM antibody, Notum antibody

Background: Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the alpha/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.

Gene ID: 77583

NCBI Accession: NP_780472

UniProt: Q8R116