Janus Kinase 2 (JAK2) (C-Term) antibody

Details for Product No. ABIN265197
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Antigen
Synonyms JAK2bCA, jak2, JTK10, THCYT3, AI504024, C81284, Fd17, JAK-2
Epitope
C-Term
(40), (29), (15), (13), (12), (10), (9), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(210), (157), (151), (38), (25), (24), (12), (2), (2), (1), (1)
Host
Rabbit
(212), (11), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application
Western Blotting (WB)
(147), (94), (91), (35), (30), (18), (17), (11), (6), (4), (3), (3), (2)
Pubmed 12 references available
Quantity 0.1 mg
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Catalog No. ABIN265197
643.50 $
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Immunogen Chemically synthesized peptide derived from the C-terminal region of human JAK2
Isotype IgG
Specificity This antibody detects JAK2. Jak2 is expressed in blood, bone marrow and lymph node.
Purification Affinity chromatography
Alternative Name Tyrosine-Protein Kinase JAK2
Background Janus Activating Kinase 2 (JAK2) is a 130 kDa tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. The JAK2 antibody is useful as a positive control and for measuring total JAK2 protein levels to compare signals obtained with the JAK2 PSSA.Synonyms: JAK-2, Janus kinase 2
Gene ID 3717
NCBI Accession NP_004963
UniProt O60674
Research Area Signaling, Cytokines, Receptors, Kinases/Phosphatases
Application Notes Western blot: 0.5-1.0 μg/mL. Positive control: Human epidermoid carcinoma (A431) cells, 3T3-L1 adipocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline, pH 7.3, with 1.0 mg/mL BSA and 0.05 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
Supplier Images
anti-Janus Kinase 2 (JAK2) (C-Term) antibody anti-Janus Kinase 2 (JAK2) (C-Term) antibody
Background publications Manning, DiSalvo, Stull: "Protein phosphorylation: quantitative analysis in vivo and in intact cell systems." in: Molecular and cellular endocrinology, Vol. 19, Issue 1, pp. 1-19, 1980 (PubMed).

Ihle: "STATs: signal transducers and activators of transcription." in: Cell, Vol. 84, Issue 3, pp. 331-4, 1996 (PubMed).

Amiri, Venema, Wang et al.: "Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 274, Issue 45, pp. 32382-6, 1999 (PubMed).

Eguchi, Dempsey, Frank et al.: "Activation of MAPKs by angiotensin II in vascular smooth muscle cells. Metalloprotease-dependent EGF receptor activation is required for activation of ERK and p38 MAPK but not for JNK." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7957-62, 2001 (PubMed).

Madamanchi, Li, Patterson et al.: "Reactive oxygen species regulate heat-shock protein 70 via the JAK/STAT pathway." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 21, Issue 3, pp. 321-6, 2001 (PubMed).

Madamanchi, Li, Patterson et al.: "Thrombin regulates vascular smooth muscle cell growth and heat shock proteins via the JAK-STAT pathway." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18915-24, 2001 (PubMed).

Myers, Andersen, Cheng et al.: "TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 47771-4, 2001 (PubMed).

Kirito, Watanabe, Sawada et al.: "Thrombopoietin regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol 3-kinase activation pathways." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 8329-37, 2002 (PubMed).

Sutherland, Chalupny, Schooley et al.: "UL16-binding proteins, novel MHC class I-related proteins, bind to NKG2D and activate multiple signaling pathways in primary NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 671-9, 2002 (PubMed).

Zeng, Yellaturu, Neeli et al.: "5(S)-hydroxyeicosatetraenoic acid stimulates DNA synthesis in human microvascular endothelial cells via activation of Jak/STAT and phosphatidylinositol 3-kinase/Akt signaling, leading to induction of expression of basic fibroblast growth factor 2." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 41213-9, 2002 (PubMed).

Shaw, Bencherif, Marrero: "Janus kinase 2, an early target of alpha 7 nicotinic acetylcholine receptor-mediated neuroprotection against Abeta-(1-42) amyloid." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 44920-4, 2002 (PubMed).

Frank, Mifune, Inagami et al.: "Distinct mechanisms of receptor and nonreceptor tyrosine kinase activation by reactive oxygen species in vascular smooth muscle cells: role of metalloprotease and protein kinase C-delta." in: Molecular and cellular biology, Vol. 23, Issue 5, pp. 1581-9, 2003 (PubMed).

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