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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308)

Details for Product anti-AKT1 Antibody No. ABIN265198, Supplier: Log in to see
Antigen
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pThr308
188
136
89
87
54
38
38
37
26
25
21
21
21
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16
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2
2
2
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2
2
2
2
2
2
2
2
2
2
2
2
2
1
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1
1
1
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1
1
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1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1162
542
410
44
41
37
34
32
20
18
11
6
5
4
2
2
2
1
1
Host
Rabbit
893
312
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
45
39
35
34
26
25
10
10
5
5
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3
3
3
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1
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Application
Western Blotting (WB)
975
554
446
223
143
120
117
71
63
36
25
11
11
5
3
3
3
3
2
1
1
1
1
1
1
Supplier
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Immunogen Chemically synthesized phosphopeptide derived from the region of human Akt1 that contains threonine 308.
Isotype IgG
Specificity This antibody detects Akt/PKB pThr308.
Purification Affinity chromatography
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background Akt, also known as Protein Kinase B (PKB) or RAC- , is a 60 kDa serine/threonine kinase that plays an important role in multiple biological responses including metabolism, cell survival and growth by phosphorylating many proteins including GSK-3 , caspase 9, BAD, and the Forkhead Transcription Factor. Akt is activated by PI3K, which in turn can interact with proteins like FAK (when FAK is phosphorylated at tyrosine 397), thereby linking activation to the cytoskeleton. Akt is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at threonine 308 is necessary for phosphorylation of serine 473, which is required for full activation of Akt.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blot: (1: 1000 starting dilution). Positive control: 3T3-L1 pre-adipocytes +/- PDGF treatment, HEK293 cells coexperessingRasV12 and RacL61.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer Phosphate buffered saline, pH 7.3, with 1.0 mg/mL BSA 0.05 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr308) (ABIN265198) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr308) antibody
Background publications Beffert, Morfini, Bock, Reyna, Brady, Herz: "Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49958-64, 2002 (PubMed).

Persad, Attwell, Gray, Mawji, Deng, Leung, Yan, Sanghera, Walsh, Dedhar: "Regulation of protein kinase B/Akt-serine 473 phosphorylation by integrin-linked kinase: critical roles for kinase activity and amino acids arginine 211 and serine 343." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 27462-9, 2001 (PubMed).

Nelson, Fry: "Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition." in: The Journal of biological chemistry, Vol. 276, Issue 18, pp. 14842-7, 2001 (PubMed).

Hsu, Shi, Krajewski, Renner, Fisher, Reed, Franke, Lichtenstein: "The AKT kinase is activated in multiple myeloma tumor cells." in: Blood, Vol. 98, Issue 9, pp. 2853-5, 2001 (PubMed).

Genot, Arrieumerlou, Ku, Burgering, Weiss, Kramer: "The T-cell receptor regulates Akt (protein kinase B) via a pathway involving Rac1 and phosphatidylinositide 3-kinase." in: Molecular and cellular biology, Vol. 20, Issue 15, pp. 5469-78, 2000 (PubMed).

Datta, Dudek, Tao, Masters, Fu, Gotoh, Greenberg: "Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery." in: Cell, Vol. 91, Issue 2, pp. 231-41, 1997 (PubMed).

del Peso, González-García, Page, Herrera, Nuñez: "Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt." in: Science (New York, N.Y.), Vol. 278, Issue 5338, pp. 687-9, 1997 (PubMed).

Dudek, Datta, Franke, Birnbaum, Yao, Cooper, Segal, Kaplan, Greenberg: "Regulation of neuronal survival by the serine-threonine protein kinase Akt." in: Science (New York, N.Y.), Vol. 275, Issue 5300, pp. 661-5, 1997 (PubMed).

Franke, Kaplan, Cantley: "PI3K: downstream AKTion blocks apoptosis." in: Cell, Vol. 88, Issue 4, pp. 435-7, 1997 (PubMed).

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